SEARCH

SEARCH BY CITATION

FilenameFormatSizeDescription
cei12126-sup-0001-si.pdf1951K

Fig. S1. Expression of cell surface activation markers on murine B cells following in-vivo poly I:C administration. C57BL/6 (B6) mice were injected intraperitoneally (i.p.) with lipopolysaccharide (LPS). On days 0 and 1 post-injection, the mice were examined for the expression of the indicated surface molecules on spleen versus hepatic B cells; n = 4 mice per group. On day 1, both liver and splenic B cells up-regulated expression of CD39, CD40, CD80 and CD86; *P < 0·05. No significant difference was observed between the liver and spleen. Data are representative of two independent experiments.

Fig. S2. Close proximity of B cells (CD19+) and dendritic cells (DCs) (CD11c+) in liver parenchyma. Cryostat sections (5 μm) of liver tissue samples from fms-like tyrosine kinase 3 ligand (Flt3L)-treated B6 mice were stained for B cells (CD19; green), DCs (CD11c; red) and nuclei (DRAQ5; blue). Fluorescent images were captured with an Olympus Fluoview 1000 confocal microscope (software version 1·7a).

Fig. S3. Splenic, but not hepatic, B cells inhibit the activation of liver myeloid dendritic cells (mDCs) in response to lipopolysaccharide (LPS) in vitro. B cell-depleted liver non-parenchymal cells (NPC) isolated from fms-like tyrosine kinase 3 ligand (Flt3L)-treated B6 mice were cultured with or without LPS in the presence or absence of hepatic or splenic B cells for 48 h. Activation of mDCs was analysed by expression of CD80, CD86 and programmed cell death 1 ligand 1 (PD-L1) on CD19B220CD3CD11c+ mDCs. Liver mDCs in the presence of B cells were compared with those in the absence of B cells; **P < 0·01.

Please note: Wiley Blackwell is not responsible for the content or functionality of any supporting information supplied by the authors. Any queries (other than missing content) should be directed to the corresponding author for the article.