Fig. S1. Immunohistochemical staining of Toll-like receptor (TLR)-3 in colonic biopsy from actively inflamed mucosa in ulcerative colitis and three different types of negative controls in the same experiment are shown. We used serial sections from same biopsy. The first negative control was made by omitting primary antibody (no ab). In the second negative control, primary antibody was replaced by isotype immunoglobulin (Ig)G1 directed against non-human epitope (IgG1). The third control was made by blocking primary antibody with recombinant human TLR-3 peptide (rhTLR-3+TLR-3ab); ×10 and ×40 magnification for each slide are shown.

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