Fig. S1. Cytospins of granulocytic/neutrophilic myeloid-derived suppressor cells (Gr-MDSCs). Isolated neutrophils or Gr-MDSCs were fixed with cytospin centrifugation and stained with May–Gruenwald–Giemsa. Images were acquired on a Carl Zeiss Fotomicroscope (×40 Planapo oil objective, Carl Zeiss) using a Canon EOS 500 camera (Canon) and Adobe Photoshop CS3 software (Adobe).

Fig. S2. Granulocytic/neutrophilic myeloid-derived suppressor cells (Gr-MDSCs) in cord blood and different age groups. Linear scale illustration of human Gr-MDSC numbers in cord blood and different age groups of healthy children and adults.

Fig. S3. Immunophenotyping of magnetic-activated cell sorting (MACS)-isolated human cord blood granulocytic/neutrophilic myeloid-derived suppressor cells (Gr-MDSCs).

The immunophenotype of MACS-isolated cord blood Gr-MDSCs is CD66bhighCD33high CD11bhighIL-4RαinterHLA-DRnegCD14neg. Representative histograms are shown.

Fig. S4. Interleukin (IL)-13 secretion of magnetic-activated cell sorting (MACS)-isolated human cord blood polymorphonuclear leucocytes (PMN) and granulocytic/neutrophilic myeloid-derived suppressor cells (Gr-MDSCs). IL-13 quantification was performed in culture supernatants of MACS-isolated human cord blood PMN and Gr-MDSCs cultured over 4 days in complete medium using standard enzyme-linked immunosorbent assay (ELISA) technique.

Table S1. Primer sequences for quantitative reverse transcription–polymerase chain reaction (qRT–PCR).

Table S2. RNA expression of (MDSC)-related genes. Quantitative reverse transcription–polymerase chain reaction (qRT–PCR) was performed in magnetic-activated cell sorting (MACS)-isolated granulocytic/neutrophilic myeloid-derived suppressor cells (Gr-MDSCs). Ct values of the housekeeping gene β-actin and 10 established MDSC-related genes are shown (higher Ct value implies lower gene expression).

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