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cei12151-sup-0001-fig_s1.pdf348K

Fig. S1. Similar patterns of CD146 co-expression with other markers after distinguishing CD3+ T cell subsets by either CD4 or CD8 staining. Peripheral blood mononuclear cells (PBMCs) from a systemic lupus erythematosus (SLE) patient were stained for CD146 and a panel other markers (‘Antigen X’). (a) CD4 T cells were gated either as CD3+CD4+ or CD3+CD8 lymphocytes. Frequencies of CD146+ CD4 cells with or without Antigen X were then enumerated. (b) The same analysis performed for CD8 T cells, which were gated either as CD3+CD4 or CD3+CD8+ lymphocytes. In both subsets, closely similar expression patterns were obtained with either gating procedure.

cei12151-sup-0002-fig_s2.pdf376K

Fig. S2. No effect of cryopreservation on patterns of CD146 versus CD45RO expression on T cells. Analysis of three systemic lupus erythematosus (SLE) patients. (a) Representative dot-plots from one patient, gated on CD4+ or CD4 T cells. (b) Percentages of indicated subpopulations in three patients. The CD4+/CD4 ratio was also unaffected by cryopreservation.

cei12151-sup-0003-fig_s3.pdf233K

Fig. S3. Surface CD146 versus intracellular forkhead box protein 3 (FoxP3) expression in gated CD4+ and CD8 peripheral blood T cells from a representative HD (of five analysed).

cei12151-sup-0004-fig_s4.pdf402K

Fig. S4. CD146 versus human leucocyte antigen D-related (HLA-DQ) [major histocompatibility complex (MHC) class II] expression in peripheral blood CD4+ and CD4 (CD8) T cells from healthy donors (HDs) and connective tissue disease (CTD) patients, analysed as in Fig. 4 of the main paper.

cei12151-sup-0005-fig_s5.pdf353K

Fig. S5. CD146 versus CD70 expression, analysed as in Fig. 4 of the main paper.

cei12151-sup-0006-fig_s6.pdf351K

Fig. S6. CD146 versus CD45RA expression in T cells from healthy donors (HDs) and systemic lupus erythematosus (SLE) patients, analysed as in Fig. 4 of the main paper. #Indicates a single donor in whom carryover of CD3− antigen-presenting cells (APC) from an adjacent well caused 100% of T cells to be aberrantly positive for CD146.

cei12151-sup-0007-fig_s7.pdf244K

Fig. S7. CXCR3 expression in total versus CD146+ CD4 and CD8 T cells from healthy donors (HDs) and systemic lupus erythematosus (SLE) patients; paired analysis as in Fig. 4b,c of the main paper (P > 0·05, not significant).

cei12151-sup-0008-fig_s8.pdf387K

Fig. S8. CD146 versus CD31 expression, analysed as in Fig. 4 of the main paper.

cei12151-sup-0009-fig_s9.pdf314K

Fig. S9. CD146 versus CD54/intercellular adhesion molecule 1 (ICAM-1) expression, analysed in healthy donors (HDs) and systemic lupus erythematosus (SLE) patients, as in Fig. 4 of the main paper.

cei12151-sup-0010-fig_s10.pdf281K

Fig. S10. CD146+ lymphocytes greatly outnumber CD146+ circulating endothelial cells. Peripheral blood mononuclear cells (PBMCs) from a healthy donor were co-stained for CD45 (leucocyte common antigen), CD146 and CD34 (the latter is expressed both on haematopoietic progenitors and on endothelial cells). Numbers represent percentages or frequencies. In the CD45+ leucocyte gate a proportion of cells stained for either CD146 or CD34, but not both. In the CD45 gate, a small number of CD34+CD146+ double-positive events were detected, which may be circulating endothelial cells (versus one event detected in isotype control).

cei12151-sup-0011-table_s1.pdf42K

Table S1. Clinical characteristics of patients.

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