B6.g7 mice reconstituted with BDC2·5 non-obese diabetic (BDC2·5NOD) stem cells do not develop autoimmune diabetes
Article first published online: 8 SEP 2013
© 2013 British Society for Immunology
Clinical & Experimental Immunology
Volume 174, Issue 1, pages 27–37, October 2013
How to Cite
Rajasekaran, N., Wang, N., Hang, Y., Macaubas, C., Rinderknecht, C., Beilhack, G. F., Shizuru, J. A. and Mellins, E. D. (2013), B6.g7 mice reconstituted with BDC2·5 non-obese diabetic (BDC2·5NOD) stem cells do not develop autoimmune diabetes. Clinical & Experimental Immunology, 174: 27–37. doi: 10.1111/cei.12163
- Issue published online: 8 SEP 2013
- Article first published online: 8 SEP 2013
- Accepted manuscript online: 25 JUN 2013 03:41AM EST
- Manuscript Accepted: 18 JUN 2013
- Arthritis Foundation
- Juvenile Diabetes Research Foundation
- American College of Rheumatology Research and Education Foundation
- NIH. Grant Numbers: AI075253, DK079163, DK067559
- H. L. Snyder Medical Foundation
- Stinehart/Reed Diabetes Research
- Stanford NIH/NCRR CTSA. Grant Number: UL1 RR025744
- Lucile Packard Foundation for Children's Health
Fig. S1. (a) Expression of congenic markers CD45·1 and CD45·2 in mice. Spleen cells from B6.g7 (left) and BDC2·5non-obese diabetic (NOD) mice (right) were stained with antibodies specifically against CD45·2 or CD45·1 and analysed by flow cytometry. (b) CD44 expression on host (black bar) and donor-derived (white bar) CD4+CD25–forkhead box protein 3 (FoxP3)– cells in the thymus of BDC2·5NODB6.g7 chimeric mice killed 12 weeks post-reconstitution. (c) Frequency of CD4+CD25+FoxP3+ cells in the host (black bar) and donor-derived (white bar) thymocytes of the BDC2·5NODB6.g7 chimeras killed 12 weeks post-transplant. Frequency shown as mean ± standard error of the mean, n = 3; **P < 0·006 (t-test).
Table S1. Antibody panel for haematopoietic stem and progenitor cell (HSPC) isolation.
Table S2. Antibody panel for fluorescence activated cell sorter (FACS) analysis.
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