Fig. S1.  (a) Expression of congenic markers CD45·1 and CD45·2 in mice. Spleen cells from B6.g7 (left) and BDC2·5non-obese diabetic (NOD) mice (right) were stained with antibodies specifically against CD45·2 or CD45·1 and analysed by flow cytometry. (b) CD44 expression on host (black bar) and donor-derived (white bar) CD4+CD25forkhead box protein 3 (FoxP3) cells in the thymus of BDC2·5NOD[RIGHTWARDS ARROW]B6.g7 chimeric mice killed 12 weeks post-reconstitution. (c) Frequency of CD4+CD25+FoxP3+ cells in the host (black bar) and donor-derived (white bar) thymocytes of the BDC2·5NOD[RIGHTWARDS ARROW]B6.g7 chimeras killed 12 weeks post-transplant. Frequency shown as mean ± standard error of the mean, n = 3; **P < 0·006 (t-test).

Table S1.  Antibody panel for haematopoietic stem and progenitor cell (HSPC) isolation.

Table S2.  Antibody panel for fluorescence activated cell sorter (FACS) analysis.

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