CD3ζ-based chimeric antigen receptors mediate T cell activation via cis- and trans-signalling mechanisms: implications for optimization of receptor structure for adoptive cell therapy
Article first published online: 3 JAN 2014
© 2013 The Authors. Clinical and Experimental Immunology published by John Wiley & Sons Ltd on behalf of British Society. for Immunology.
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Clinical & Experimental Immunology
Volume 175, Issue 2, pages 258–267, February 2014
How to Cite
Bridgeman, J. S., Ladell, K., Sheard, V. E., Miners, K., Hawkins, R. E., Price, D. A. and Gilham, D. E. (2014), CD3ζ-based chimeric antigen receptors mediate T cell activation via cis- and trans-signalling mechanisms: implications for optimization of receptor structure for adoptive cell therapy. Clinical & Experimental Immunology, 175: 258–267. doi: 10.1111/cei.12216
- Issue published online: 3 JAN 2014
- Article first published online: 3 JAN 2014
- Accepted manuscript online: 9 OCT 2013 12:00AM EST
- Manuscript Accepted: 29 SEP 2013
- BBSRC Studentship and LoLa Grant. Grant Number: BB/H001085/1
- Wellcome Trust
- Cancer Research UK
- European Commission FP6 programme ATTACK
Fig. S1. Immunoreceptor tyrosine-based activation motif (ITAM)-truncated chimeric antigen receptors retain functional capacity in primary human T cells – T cells from three healthy donors were transduced with the indicated chimeric antigen receptors (CARs) and expanded in vitro. Transduced CD4+ and CD8+ cells, identified by FLAG expression, were assessed for CD107a mobilization and intracellular production of interferon (IFN)-γ, tumour necrosis factor (TNF)-α and interleukin (IL)-2 after stimulation for 6 h with the indicated concentrations of immobilized carcinoembryonic antigen (CEA) protein.
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