Both authors contribute equally to this study.
CD4+CD25highforkhead box protein 3+ regulatory T lymphocytes suppress interferon-γ and CD107 expression in CD4+ and CD8+ T cells from tuberculous pleural effusions
Article first published online: 3 JAN 2014
© 2013 British Society for Immunology
Clinical & Experimental Immunology
Volume 175, Issue 2, pages 235–245, February 2014
How to Cite
Geffner, L., Basile, J. I., Yokobori, N., Sabio y García, C., Musella, R., Castagnino, J., Sasiain, M. C. and de la Barrera, S. (2014), CD4+CD25highforkhead box protein 3+ regulatory T lymphocytes suppress interferon-γ and CD107 expression in CD4+ and CD8+ T cells from tuberculous pleural effusions. Clinical & Experimental Immunology, 175: 235–245. doi: 10.1111/cei.12227
- Issue published online: 3 JAN 2014
- Article first published online: 3 JAN 2014
- Accepted manuscript online: 18 OCT 2013 12:00AM EST
- Manuscript Accepted: 15 OCT 2013
- Agencia Nacional de Promoción Científica y Tecnológica. Grant Numbers: PAE-PICT 07-2328, PAE-PICT 07-2329, PICT 2010-0599
- pleural effusions;
- T regulatory;
Tuberculous pleural effusion is characterized by a T helper type 1 (Th1) profile, but an excessive Th1 response may also cause tissue damage that might be controlled by regulatory mechanisms. In the current study we investigated the role of regulatory T cells (Treg) in the modulation of Th1 responses in patients with tuberculous (TB) pleurisy. Using flow cytometry we evaluated the proportion of Treg (CD4+CD25highforkhead box protein 3+), interferon (IFN)-γ and interleukin (IL)-10 expression and CD107 degranulation in peripheral blood (PB) and pleural fluid (PF) from patients with TB pleurisy. We demonstrated that the proportion of CD4+CD25+, CD4+CD25highFoxP3+ and CD8+CD25+ cells were increased in PF compared to PB samples. Mycobacterium tuberculosis stimulation increased the proportion of CD4+CD25low/negIL-10+ in PB and CD4+CD25low/negIFN-γ+ in PF; meanwhile, CD25high mainly expressed IL-10 in both compartments. A high proportion of CD4+CD107+ and CD8+CD107+ cells was observed in PF. Treg depletion enhanced the in-vitro M. tuberculosis-induced IFN-γ and CD4+ and CD8+ degranulation responses and decreased CD4+IL-10+ cells in PF. Our results demonstrated that in TB pleurisy Treg cells effectively inhibit not only IFN-γ expression but also the ability of CD4+ and CD8+ cells to degranulate in response to M. tuberculosis.