Fig. S1. Verification of DNA vector-driven antigen expression in mammalian cells was verified exemplarily for four secretory granule antigens and six non-secretory granule antigens. Lysates of human embryonic kidney (HEK) 293 cells transfected with pcDNA 3·1 V5HISB DNA vectors encoding the indicated proteins were separated via sodium dodecyl sulphate-polyacrylamide gel electrophoresis (SDS-PAGE) and analysed using immunoblotting with V5-tag directed antibodies. Equal amounts of lysates were loaded. Blots show empty vector-transfected controls (left lanes, −) and antigen-transfected samples (right lanes, +) for each antigen. Protein standards in kDa are shown for each respective sample.

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