These authors contributed equally to the work.
IGRP and insulin vaccination induce CD8+ T cell-mediated autoimmune diabetes in the RIP-CD80GP mouse
Version of Record online: 9 APR 2014
© 2014 British Society for Immunology
Clinical & Experimental Immunology
Volume 176, Issue 2, pages 199–206, May 2014
How to Cite
Fuchs, Y. F., Adler, K., Lindner, A., Karasinsky, A., Wilhelm, C., Weigelt, M., Balke, H., Förtsch, K., Mortler-Hildebrandt, L. F., Harlan, D. M., Pechhold, K., Ziegler, A.-G. and Bonifacio, E. (2014), IGRP and insulin vaccination induce CD8+ T cell-mediated autoimmune diabetes in the RIP-CD80GP mouse. Clinical & Experimental Immunology, 176: 199–206. doi: 10.1111/cei.12263
- Issue online: 9 APR 2014
- Version of Record online: 9 APR 2014
- Accepted manuscript online: 6 JAN 2014 05:49AM EST
- Manuscript Accepted: 27 DEC 2013
- German Federal Ministry of Education and Research (BMBF)
- DFG Research Center and Cluster of Excellence – Center for Regenerative Therapies Dresden. Grant Number: FZ 111
- NIH/DFG Research Career Transition Award Program. Grant Number: KO 3418/1-1
- Das zuckerkranke Kind
Fig. S1. Verification of DNA vector-driven antigen expression in mammalian cells was verified exemplarily for four secretory granule antigens and six non-secretory granule antigens. Lysates of human embryonic kidney (HEK) 293 cells transfected with pcDNA 3·1 V5HISB DNA vectors encoding the indicated proteins were separated via sodium dodecyl sulphate-polyacrylamide gel electrophoresis (SDS-PAGE) and analysed using immunoblotting with V5-tag directed antibodies. Equal amounts of lysates were loaded. Blots show empty vector-transfected controls (left lanes, −) and antigen-transfected samples (right lanes, +) for each antigen. Protein standards in kDa are shown for each respective sample.
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