Cytomegalovirus drives Vδ2neg γδ T cell inflation in many healthy virus carriers with increasing age
Article first published online: 24 APR 2014
© 2014 British Society for Immunology
Clinical & Experimental Immunology
Volume 176, Issue 3, pages 418–428, June 2014
How to Cite
Alejenef, A., Pachnio, A., Halawi, M., Christmas, S. E., Moss, P. A. H. and Khan, N. (2014), Cytomegalovirus drives Vδ2neg γδ T cell inflation in many healthy virus carriers with increasing age. Clinical & Experimental Immunology, 176: 418–428. doi: 10.1111/cei.12297
- Issue published online: 24 APR 2014
- Article first published online: 24 APR 2014
- Accepted manuscript online: 19 FEB 2014 01:03AM EST
- Manuscript Accepted: 10 FEB 2014
- Government of Libya. Grant Number: SL014-611-18202
- a Wellcome Trust Value In People Award. Grant Number: 078660/Z/05/Z
Fig. S1. Flow cytometry plots showing peripheral blood mononuclear cells (PBMC) stained with pan-T cell receptor (TCR)-γδ and anti-Vδ1 or anti-Vδ2 monoclonal antibodies from two cytomegalovirus (CMV)-seronegative (a) and two CMV-seropositive healthy donors (b). Values shown in the two upper quadrants of each plot indicate the percentage of lymphocytes staining for each γδ subset.
Fig. S2. Analysis of memory Vδ2neg γδ T-cells in cytomegalovirus (CMV)-seropositive (a) and CMV-seronegative donors (b) of different age groups. Charts show the absolute numbers of memory Vδ2neg γδ T cells in subjects as determined by co-staining with CD45RA and lymphocyte function-associated antigen 1 (LFA-1). Memory cells comprised both T effector memory (Tem) and T effector memory CD45RA-positive (TemRA) cells (see Fig. 2).
Please note: Wiley Blackwell is not responsible for the content or functionality of any supporting information supplied by the authors. Any queries (other than missing content) should be directed to the corresponding author for the article.