Fig. S1. Flow cytometry plots showing peripheral blood mononuclear cells (PBMC) stained with pan-T cell receptor (TCR)-γδ and anti-Vδ1 or anti-Vδ2 monoclonal antibodies from two cytomegalovirus (CMV)-seronegative (a) and two CMV-seropositive healthy donors (b). Values shown in the two upper quadrants of each plot indicate the percentage of lymphocytes staining for each γδ subset.

Fig. S2. Analysis of memory Vδ2neg γδ T-cells in cytomegalovirus (CMV)-seropositive (a) and CMV-seronegative donors (b) of different age groups. Charts show the absolute numbers of memory Vδ2neg γδ T cells in subjects as determined by co-staining with CD45RA and lymphocyte function-associated antigen 1 (LFA-1). Memory cells comprised both T effector memory (Tem) and T effector memory CD45RA-positive (TemRA) cells (see Fig. 2).

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