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cei12312-sup-0001-si.tif38K

Fig S1. Scheme of immunoglobulin (Ig)G and F(ab)2 purification, used in the current study.

cei12312-sup-0002-si.tif193K

Fig S2. Abzymes obtained after purification with ammonium sulphate precipitation, protein-G affinity chromatography and size exclusion-high performance liquid chromatography (HPLC-SEC) are essentially pure, as can be seen for rabbit abzymes at (a) further fractionated using HPLC- strong cation exchange (SCX) in the NaCl gradient and subsequent measurement of sialidase activity of each fraction; they are also not contaminated with immune complexes, as demonstrated for systemic lupus erythematosus (SLE) abzyme at (b) during HPLC-SEC at acidic conditions, pH 2·6.

cei12312-sup-0003-si.tif173K

Fig S3. Kinetic parameters of sialidase reaction catalyzed by sialidase-abzymes from immunized rabbits. (a) Michaelis–Menten plot; (b) Lineweaver–Burk plot: the incubation time for all samples was 180 min; (c) dose-dependent effect on reaction product [methylumbelliferole (MU)] accumulation at different amounts of abzymes in the reaction medium. Reaction was carried out at 100 μM of 4-Mu-NA for 240 min. Main kinetic constants are indicated on the Lineweaver–Burk plot.

cei12312-sup-0004-si.xls44K

Table S1. Serological features and cinical data of studied systemic lupus erythematosus (SLE) patients.

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