Innate DNA sensing is impaired in HIV patients and IFI16 expression correlates with chronic immune activation
Article first published online: 9 JUN 2014
© 2014 British Society for Immunology
Clinical & Experimental Immunology
Volume 177, Issue 1, pages 295–309, July 2014
How to Cite
Nissen, S. K., Højen, J. F., Andersen, K. L. D., Kofod-Olsen, E., Berg, R. K., Paludan, S. R., Østergaard, L., Jakobsen, M. R., Tolstrup, M. and Mogensen, T. H. (2014), Innate DNA sensing is impaired in HIV patients and IFI16 expression correlates with chronic immune activation. Clinical & Experimental Immunology, 177: 295–309. doi: 10.1111/cei.12317
- Issue published online: 9 JUN 2014
- Article first published online: 9 JUN 2014
- Accepted manuscript online: 5 MAR 2014 04:52AM EST
- Manuscript Accepted: 28 FEB 2014
- Danish Medical Research Council. Grant Numbers: 09-066031, 12-124330, 10-081986
- Kong Christian IX
- Aase og Ejnar Danielsens Fond
- Dronning Louises Jubilæumslegat
- The Foundation for the Advancement of Medical Sciences
Fig. S1. Immune activation in CD4+ and CD4– T cell populations from the different study groups. CD3+ T cells were analysed for CD38 surface expression as a marker of activation. (a) CD4+ cells, (b) CD4– cells. Data are presented as dot-plots with each dot representing one patient; n.s. = non-significant. P-values < 0.05 were considered significant.
Fig. S2. Immune activation in different T cell subsets correlated to viral load and expression of DNA sensors. CD3+ T cells were subdivided into the following subsets by flow cytometry: naive T cells (CD45RA+CD27+CCR7+), central memory T cells (CD45RA–CD27+CCR7+), effector memory T cells (CD45RA–CD27+CCR7–) and terminally differentiated T cells (CD45RA+CD27–CCR7–). CD38 surface expression levels are correlated with viral load in CD4+ (a) and CD4– (b) T cell subsets, respectively. CD38 surface expression levels are correlated with interferon-inducible protein 16 (IFI16) mRNA expression in the CD4+ (c) and CD4– (d) T cell subsets, respectively. Pearson correlations, P-values and significance levels are shown in Table 2.
Table S1. Overview of highly active anti-retroviral therapy (HAART) regimens in HAART responders and immunological non-responders (INRs).
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