Impaired T cell activation and cytokine production by calcitriol-primed human B cells
Version of Record online: 1 OCT 2014
© 2014 British Society for Immunology
Clinical & Experimental Immunology
Volume 178, Issue 2, pages 364–372, November 2014
How to Cite
Drozdenko, G., Scheel, T., Heine, G., Baumgrass, R. and Worm, M. (2014), Impaired T cell activation and cytokine production by calcitriol-primed human B cells. Clinical & Experimental Immunology, 178: 364–372. doi: 10.1111/cei.12406
- Issue online: 1 OCT 2014
- Version of Record online: 1 OCT 2014
- Accepted manuscript online: 26 JUN 2014 04:32AM EST
- Manuscript Accepted: 22 JUN 2014
- Deutsche Forschungsgemeinschaft. Grant Number: DFG – SFB650/TP5
Fig. S1. T cell proliferation following 7-day co-culture with activated B cells. Carboxyfluorescein diacetate N-succinimidylester (CFSE) dilution was determined in CD4+ T cells after 7-day co-culture activated [anti-CD40, 1 μg/ml + interleukin (IL)-4, 10 ng/ml, for 48 h] B cells. (a) T cell proliferation detected upon 7-day co-culture with activated and calcitriol primed, as indicated in the figure, B cells. (b) T cell proliferation upon 7-day co-culture with activated B cells in the presence of increasing concentrations of toxic shock syndrome toxin 1 (TSST-1). Data represent four to five independent experiments and analysed using the analysis of variance (anova) test. Data are shown as mean ± standard deviation. *P ≤ 0·05; **P ≤ 0·01; ***P ≤ 0·001 are considered significant.
Fig. S2. No relevant calcitriol spillover from B cell culture into T–B cell co-culture. The human promyelocytic leukaemia HL-60 cell line was incubated with calcitriol 1 μmol/l–100 pmol/l (upper plots) or supernatants from anti-CD40 (1 μg/ml), interleukin (IL)-4 (10 ng/ml) preactivated and calcitriol (as indicated in the Figure)-treated B cells; 48 h preactivation following washing and additional incubation with RPMI-1640 medium for 1 and 3 days. Supernatants were tested for calcitriol spillover with calcitriol-dependent expression of CD38 by the HL-60 cell line.
Table S1. Primer sequences for quantitative polymerase chain reaction (qPCR).
Please note: Wiley Blackwell is not responsible for the content or functionality of any supporting information supplied by the authors. Any queries (other than missing content) should be directed to the corresponding author for the article.