These authors contributed equally to this work.
Unconventional apoptosis of polymorphonuclear neutrophils (PMN): staurosporine delays exposure of phosphatidylserine and prevents phagocytosis by MΦ-2 macrophages of PMN
Version of Record online: 26 NOV 2014
© 2014 British Society for Immunology
Clinical & Experimental Immunology
Volume 179, Issue 1, pages 75–84, January 2015
How to Cite
Franz, S., Muñoz, L. E., Heyder, P., Herrmann, M. and Schiller, M. (2015), Unconventional apoptosis of polymorphonuclear neutrophils (PMN): staurosporine delays exposure of phosphatidylserine and prevents phagocytosis by MΦ-2 macrophages of PMN. Clinical & Experimental Immunology, 179: 75–84. doi: 10.1111/cei.12412
- Issue online: 26 NOV 2014
- Version of Record online: 26 NOV 2014
- Accepted manuscript online: 3 JUL 2014 09:19PM EST
- Manuscript Accepted: 1 JUL 2014
- Deutsche Forschungsgemeinschaft. Grant Numbers: DFG SFB-TR67, project B3, DFG SFB-643, project B5, Schi 1006/5-1, Lo 437/9-1
Fig. S1. Gating strategy to determine the amount of cells within the populations of full size polymorphonuclear neutrophils (PMN) (P1), of shrunken PMN (P2) and of fragments (P3). Percentage of each population within PMN undergoing apoptosis was calculated as follows: full size [P1/(P1 + P2) × 100]; shrunken [P2/(P1 + P2) × 100]; fragments [P3/(P1 + P2 + P3) × 100].
Table S1. In staurosporine-treated polymorphonuclear neutrophils (PMN) apoptosis-related surface alterations are modified. Apoptosis in PMN was induced by stimulation with different doses (10 nM, 100 nM, 1000 nM) of staurosporine (STS) and by in-vitro culture for 22 h (spontaneous) or prevented by activation with granulocyte-colony-stimulating factor (G-CSF) (10 000U/ml). After 20 h surface alterations were determined by analysing expression of surface markers CD11a, CD16, CD32, CD62L, CD89 and binding of lectins NPn, GSL II, UEA I. Compared to PMN undergoing spontaneous apoptosis, expression of neo-surface antigens is enhanced in STS-treated PMN, whereas loss of surface proteins is similar. Data are mean percentage (± standard deviation) of antibody/lectin binding relative to fresh isolated PMN (set to 100%) of duplicates of one representative experiment of three independent experiments.
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