Fig. S1. Gating strategy to determine the amount of cells within the populations of full size polymorphonuclear neutrophils (PMN) (P1), of shrunken PMN (P2) and of fragments (P3). Percentage of each population within PMN undergoing apoptosis was calculated as follows: full size [P1/(P1 + P2) × 100]; shrunken [P2/(P1 + P2) × 100]; fragments [P3/(P1 + P2 + P3) × 100].

Table S1. In staurosporine-treated polymorphonuclear neutrophils (PMN) apoptosis-related surface alterations are modified. Apoptosis in PMN was induced by stimulation with different doses (10 nM, 100 nM, 1000 nM) of staurosporine (STS) and by in-vitro culture for 22 h (spontaneous) or prevented by activation with granulocyte-colony-stimulating factor (G-CSF) (10 000U/ml). After 20 h surface alterations were determined by analysing expression of surface markers CD11a, CD16, CD32, CD62L, CD89 and binding of lectins NPn, GSL II, UEA I. Compared to PMN undergoing spontaneous apoptosis, expression of neo-surface antigens is enhanced in STS-treated PMN, whereas loss of surface proteins is similar. Data are mean percentage (± standard deviation) of antibody/lectin binding relative to fresh isolated PMN (set to 100%) of duplicates of one representative experiment of three independent experiments.

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