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cge12141-sup-0001-FigureS1.tiffTIFF image1522KFigure S1. (a) Conserved residues. The conserved residues of Tubb2b that are in close proximity to GDP are colored green/red. Cα backbone, gray; GDP, magenta with Van der Waals surface, red marks residues in contact with GDP. (b) Partial peptide sequence alignment of human Tubb2b (NP_821080) with FtsZ protein from Methanocaldococcus jannaschii (Q57816), Staphylococcus aureus (P0A031) and Escherichia coli (P0A9A6). Numbers pertain to human Tubb2b sequence. Residues that are in contact with the GDP molecule are colored in red. (c) Box plot of root mean square deviation (RMSD) values for mutated vs wild-type (1TUB, B chain) protein. Three proposed mutated forms of human Tubb2b (two models from SWISS-MODEL Repository, based on 3HKE and 4DRX, one model from I-TASSER, Job ID S109651) have been compared in means of overall RMSD (>300 residues involved), RMSD of the conserved residues depicted in (b) and the geometric mean of RMSD of two random patches upstream and downstream of the conserved sequence (Arg251-Phe260, Met299-Asp304 and Asn14-Val23, Arg62-Asp67) to avoid bias.

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