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Clinical Oral Implants Research

Bacterial adhesion on the titanium and zirconia abutment surfaces

Authors

  • Cássio do Nascimento,

    Corresponding author
    1. Department of Dental Materials and Prosthodontics, Faculty of Dentistry of Ribeirão Preto, University of São Paulo, São Paulo, Brazil
    • Corresponding author:

      Dr Cássio do Nascimento

      Faculdade de Odontologia de Ribeirão Preto, Universidade de São Paulo, Av. do Café s/n, CEP: 14040-904 Ribeirão Preto, São Paulo, Brazil

      Tel.: +55 16 3602 4095

      Fax: +55 16 3602 0547

      e-mail: cassionasc@forp.usp.br

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  • Murillo Sucena Pita,

    1. Department of Dental Materials and Prosthodontics, Faculty of Dentistry of Ribeirão Preto, University of São Paulo, São Paulo, Brazil
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  • Flávio Henrique Nogueira Carriço Fernandes,

    1. Department of Dental Materials and Prosthodontics, Faculty of Dentistry of Ribeirão Preto, University of São Paulo, São Paulo, Brazil
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  • Vinícius Pedrazzi,

    1. Department of Dental Materials and Prosthodontics, Faculty of Dentistry of Ribeirão Preto, University of São Paulo, São Paulo, Brazil
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  • Rubens Ferreira de Albuquerque Junior,

    1. Department of Dental Materials and Prosthodontics, Faculty of Dentistry of Ribeirão Preto, University of São Paulo, São Paulo, Brazil
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  • Ricardo Faria Ribeiro

    1. Department of Dental Materials and Prosthodontics, Faculty of Dentistry of Ribeirão Preto, University of São Paulo, São Paulo, Brazil
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Abstract

Objective

Microorganisms harboring the oral cavity, mainly those related to periodontal diseases, are the most potential etiologic factor of failure in long-term implant treatment. The material used for abutment components may influence the adhesion and colonization of microbial species. The aim of this in vivo investigation was to evaluate the biofilm formation on machined (MPT) or cast titanium (CPT) and zirconia abutments (Zc).

Methods

Six healthy subjects were enrolled in this randomized crossover clinical investigation. The study was conducted in three phases according to abutment surface evaluated. Each subject used an individual oral splint containing four disks of the same tested substrate, two located in the anterior and two in the posterior region, totalizing 12 specimens for subject. Participants were asked to use the removable intraoral splint during 24 h. DNA checkerboard hybridization method was used to identify and quantify 38 bacterial species colonizing formed biofilm on the abutment substrates.

Results

Pathogens and non-pathogens species were found colonizing the three substrates surfaces. Fusobacterium nucleatum, Neisseria mucosa, Porphyromonas aeruginosa, Peptostreptococcus anaerobios, Staphylococcus aureus, Streptococcus gordonii, Streptococcus parasanguinis, and Tanerella forsythia were the only species with no significant differences over the tested materials (> 0.05). All the other target species presented significant differences sought by Friedman test (< 0.0001).

Conclusions

There was a significant difference in the total bacterial count between the three groups. CPT presented the higher mean counts, followed by MPT and Zc. CPT group also showed a higher mean incidence of species than MPT and Zc. The anterior or posterior region of disks placement did not show significant differences in relation to bacterial adhesion.

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