Osteoblastic and osteoclastic differentiation on SLA and hydrophilic modified SLA titanium surfaces

Authors

  • Sung-Moon Bang,

    1. Department of Oral & Maxillofacial Surgery, Institute of Oral Biology, Kyung Hee University School of Dentistry, Seoul, Korea
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    • Equally contributed to this work.
  • Ho-Jin Moon,

    1. Department of Maxillofacial Biomedical Engineering, Institute of Oral Biology, Kyung Hee University School of Dentistry, Seoul, Korea
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    • Equally contributed to this work.
  • Yong-Dae Kwon,

    Corresponding author
    1. Department of Oral & Maxillofacial Surgery, Institute of Oral Biology, Kyung Hee University School of Dentistry, Seoul, Korea
    • Corresponding author:

      Yong-Dae Kwon, DMD, MSD, PhD

      Associate Professor, Department of Oral & Maxillofacial Surgery, Institute of Oral Biology, School of Dentistry, Kyung Hee University, 26 Kyungheedae-ro, Dongdaemun-gu, Seoul 130-701, Korea

      Tel.: +82 2 958 9440

      Fax: +82 2 966 4572

      e-mail: yongdae.kwon@gmail.com

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  • Ji-Yeon Yoo,

    1. Department of Oral & Maxillofacial Surgery, Institute of Oral Biology, Kyung Hee University School of Dentistry, Seoul, Korea
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  • Ahran Pae,

    1. Department of Prosthodontics, Institute of Oral Biology, Kyung Hee University School of Dentistry, Seoul, Korea
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  • Il Keun Kwon

    1. Department of Maxillofacial Biomedical Engineering, Institute of Oral Biology, Kyung Hee University School of Dentistry, Seoul, Korea
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Abstract

Purpose

We evaluated the activities of both osteoblastic and osteoclastic differentiation on sandblasted/acid etched (SLA), hydrophilic SLA surfaces (modSLA) and pretreatment titanium (PT).

Material and methods

The osteoblastic differentiation was evaluated by alkaline phosphatase analysis and Alizarin Red S staining, and the expression of bone-related proteins, alkaline phosphatase (ALP), runt-related transcription factor 2 (Runx2), osteopontin (OPN), and osteocalcin (OCN), was investigated by reverse transcriptase–polymerase chain reaction (RT-PCR). Primary mice monocytes were expanded and differentiated in the presence of macrophage-colony stimulating factor (M-CSF), and osteoclastic differentiation was evaluated by actin ring formation assay and tartrate-resistant acid phosphatase (TRAP) activity assay. Real-time PCR tests were performed to investigate the expression of gene mRNA expression levels in osteoclast cells.

Result

Differentiation of osteoblasts in the Alizarin Red S test staining and ALP assay was significantly increased in the modSLA surface. The preceding results were supported by the result of RT-PCR for the expression of Runx2, OPN, and OCN. As for osteoclastic activity, differentiated osteoclasts rarely existed on the SLA and modSLA surface with actin ring. The results of real-time PCR and TRAP activity supported the preceding results.

Conclusion

It may be concluded that the modSLA surface promotes osteogenic effect and prevents osteoclastic differentiation. Promotion of osteoblastic proliferation after a short-term cell culture might be responsible for stimulated bone regeneration implying that early loading may be possible. Also, the anti-osteoclastic effect of the modSLA surface may contribute to maintenance of the marginal bone level of dental implants, implying long-term stability would be provided by this surface technology. The modSLA surface may not only make early loading possible but possibly reduce marginal bone loss during the maintenance phase.

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