Effects of directly autotransplanted tibial bone marrow aspirates on bone regeneration and osseointegration of dental implants

Authors

  • Michael Payer,

    Corresponding author
    1. Department of Oral Surgery and Radiology, School of Dentistry, Medical University of Graz, Graz, Austria
    • Corresponding author:

      PD Dr. Michael Payer

      Department of Oral Surgery and Radiology

      School of Dentistry, Medical University Graz

      Auenbruggerplatz 12, A-8036 Graz, Austria

      Tel.: +43 316 385 12936

      Fax: +43 316 385 16858

      e-mail: mi.payer@medunigraz.at

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    • These authors contributed equally.
  • Birgit Lohberger,

    1. Department of Orthopedic Surgery, Medical University of Graz, Graz, Austria
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    • These authors contributed equally.
  • Dirk Strunk,

    1. Department of Hematology, Stem Cell Cluster, Medical University of Graz, Graz, Austria
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  • Karoline M. Reich,

    1. Department of Oral Surgery, Karl Donath Laboratory for Hard Tissue and Biomaterial Research, Medical University of Vienna, Vienna, Austria
    2. Austrian Cluster for Tissue Regeneration, Vienna, Austria
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  • Stephan Acham,

    1. Department of Oral Surgery and Radiology, School of Dentistry, Medical University of Graz, Graz, Austria
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  • Norbert Jakse

    1. Department of Oral Surgery and Radiology, School of Dentistry, Medical University of Graz, Graz, Austria
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Abstract

Objectives

Aim of the pilot trial was to evaluate applicability and effects of directly autotransplanted tibial bone marrow (BM) aspirates on the incorporation of porous bovine bone mineral in a sinus lift model and on the osseointegration of dental implants.

Material and methods

Six edentulous patients with bilaterally severely resorbed maxillae requiring sinus augmentation and implant treatment were included. During surgery, tibial BM was harvested and added to bone substitute material (Bio-Oss®) at the randomly selected test site. At control sites, augmentation was performed with Bio-Oss® alone. The cellular content of each BM aspirate was checked for multipotency and surface antigen expression as quality control. Histomorphometric analysis of biopsies from the augmented sites after 3 and 6 months (during implantation) was used to evaluate effects on bone regeneration. Osseointegration of implants was evaluated with Periotest® and radiographic means.

Results

Multipotent cellular content in tibial BM aspirates was comparable to that in punctures from the iliac crest. No significant difference in amount of new bone formation and the integration of bone substitute particles was detected histomorphometrically. Periotest® values and radiographs showed successful osseointegration of inserted implants at all sites.

Conclusion

Directly autotransplanted tibial BM aspirates did not show beneficial regenerative effects in the small study population (N = 6) of the present pilot trial. However, the proximal tibia proved to be a potential donor site for small quantities of BM. Future trials should clarify whether concentration of tibial BM aspirates could effect higher regenerative potency.

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