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In vitro adhesion of fibroblastic cells to titanium alloy discs treated with sodium hydroxide

Authors

  • Maisa Al Mustafa,

    1. Department of Oral Surgery, Medical University of Vienna, Vienna, Austria
    2. Austrian Cluster for Tissue Regeneration, Vienna, Austria
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  • Hermann Agis,

    1. Austrian Cluster for Tissue Regeneration, Vienna, Austria
    2. Department of Conservative Dentistry and Periodontology, Medical University of Vienna, Vienna, Austria
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  • Heinz-Dieter Müller,

    1. Department of Prosthodontics, Medical University of Vienna, Vienna, Austria
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  • Georg Watzek,

    1. Department of Oral Surgery, Medical University of Vienna, Vienna, Austria
    2. Austrian Cluster for Tissue Regeneration, Vienna, Austria
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  • Reinhard Gruber

    Corresponding author
    1. Department of Oral Surgery, Medical University of Vienna, Vienna, Austria
    2. Austrian Cluster for Tissue Regeneration, Vienna, Austria
    3. Laboratory of Oral Cell Biology, School of Dental Medicine, University of Bern, Bern, Switzerland
    • Corresponding author:

      Reinhard Gruber, PhD

      Department of Oral Surgery, Bernhard Gottlieb Dental School, Medical University Vienna, Sensengasse 2a, A-1090 Vienna, Austria; and Laboratory of Oral Cell Biology, School of Dental Medicine, University of Bern, Freiburgstrasse 7, 3010 Bern, Switzerland

      Tel.:+43 1 4277 67011

      Fax: +43 1 4277 67019

      e-mails: reinhard.gruber@meduniwien.ac.at; reinhard.gruber@zmk.unibe.ch

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Abstract

Objective

Adhesion of osteogenic cells on titanium surfaces is a prerequisite for osseointegration. Alkali treatment can increase the hydrophilicity of titanium implant surfaces, thereby supporting the adhesion of blood components. However, it is unclear if alkali treatment also supports the adhesion of cells with a fibroblastic morphology to titanium.

Materials and methods

Here, we have used a titanium alloy (Ti-6AL-4V) processed by alkali treatment to demonstrate the impact of hydrophilicity on the adhesion of primary human gingival fibroblast and bone cells. Also included were the osteosarcoma and fibroblastoma cell lines, MG63 and L929, respectively. Cell adhesion was determined by scanning electron microscopy. We also measured viability, proliferation, and protein synthesis of the adherent cells.

Results

Alkali treatment increased the adhesion of gingival fibroblasts, bone cells, and the two cell lines when seeded onto the titanium alloy surface for 1 h. At 3 h, no significant changes in cell adhesion were observed. Cells grown for 1 day on the titanium alloy surfaces processed by alkali treatment behave similarly to untreated controls with regard to viability, proliferation, and protein synthesis.

Conclusion

Based on these preliminary In vitro findings, we conclude that alkali treatment can support the early adhesion of cells with fibroblastic characteristics to a titanium alloy surface.

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