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Fig. S1. Subcellular localization of the TvTSP3CT6 protein in parasites bound to VECs. Parasites transfected with TvTSP3CT6 containing a C-terminal HA tag were bound to VECs for 0.5, 1, 3 and 6 h and the protein was localized using an anti-HA antibody. Note the change in localization from plasma membrane and intracellular vesicles to primarily a flagellar localization at 3 and 6 h post host cell exposure. The red arrow indicates the flagella and the white arrow indicates intracellular vesicles and the plasma membrane of the main body of the cell. Scale bar, 10 μm.

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