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cmi12006-sup-0001-movie1.avi285K

Movie S1. Live-video microscopy of T. annulata-infected TaC12 cells. 640× magnification (cropped), 60 s intervals, 60 min recording, 600× acceleration.

cmi12006-sup-0002-movie2.m4v1527K

Movie S2. Live-video microscopy of isolated T. annulata schizonts. 640× magnification (cropped), 30 s intervals, 60 min recording, 300× acceleration.

cmi12006-sup-0003-movie 3.m4v1173K

Movie S3. Live-video microscopy of isolated T. annulata schizonts. 640× magnification (cropped), 30 s intervals, 60 min recording, 300× acceleration.

cmi12006-sup-0004-movie4.m4v2284K

Movie S4. Live-video microscopy of isolated T. annulata schizonts. 640× magnification (cropped), 30 s intervals, 120 min recording, 300× acceleration.

cmi12006-sup-0005-movie5.m4v879K

Movie S5. Isolated T. annulata schizonts were allowed to start protrusion formation for 45 min. Cytochalasin D at a concentration of 2 μM was the added and live-video microscopy was performed. 64× magnification (cropped), 30 s intervals, 45 min recording, 300× acceleration.

cmi12006-sup-0006-fS1.tif2127K

Fig. S1. Sequence alignment of actins from Toxoplasma gondii (TgACTI), Plasmodium falciparum (PfACTI) and Theileria annulata (TaACT1). Residues that were demonstrated to give instability to actin filaments (G200S and K270M) are indicated by an asterisk.

cmi12006-sup-0007-fS2.tif3215K

Fig. S2. Isolated parasites were incubated with antibody raised against Toxoplasma actin and anti-TaSP antibody. Isolated parasites were fixed immediately after purification (A) or after 1 h in medium (B) to induce membranous protrusions. Arrowheads indicate dotted anti-actin immunoreactivity in membranous protrusions. Arrowheads highlight actin dots inside membranous protrusions.

cmi12006-sup-0008-fS3.tif1247K

Fig. S3. Cryo-electron micrographs of isolated Theileria showing thin membranous protrusions (arrows) that end in a bulge-like structure (arrowhead). Some slices through the reconstructed tomograms are shown in Fig. 5. Bars = 400 nm.

cmi12006-sup-0009-fS4.tif1306K

Fig. S4. Cryo-electron micrographs of isolated Theileria showing thick membranous protrusions (arrows), presenting membranous structures inside them (arrowhead). Some slices through the reconstructed tomograms are shown in Fig. 6. Bars = 400 nm.

cmi12006-sup-0010-fS5.tif1530K

Fig. S5. Cryo-electron tomography analysis of a thick filamentous protrusion of Theileria.

A. A slice through a tomogram of a thick membranous protrusion (white arrows) of an isolated Theileria. Arrowhead indicates a membranous structure of twisted shape inside the protrusion.

B. Higher magnification of the membranous structure (arrowhead) inside the protrusion.

C. Slice through the tomogram in a different Z-projection showing some filamentous structures (black arrows).

Bars: A = 200 nm; B and C = 100 nm.

cmi12006-sup-0011-fS6.tif1696K

Fig. S6. Theileria protrusion shown in Fig. 8 after 3D modelling and rendering. The plasma membrane is in green, membranous structures in yellow and magenta, and the actin filaments in blue.

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