The arginine-rich N-terminal domain of ROP18 is necessary for vacuole targeting and virulence of Toxoplasma gondii
Version of Record online: 20 SEP 2012
© 2012 Blackwell Publishing Ltd
Volume 14, Issue 12, pages 1921–1933, December 2012
How to Cite
Fentress, S. J., Steinfeldt, T., Howard, J. C. and Sibley, L. D. (2012), The arginine-rich N-terminal domain of ROP18 is necessary for vacuole targeting and virulence of Toxoplasma gondii. Cellular Microbiology, 14: 1921–1933. doi: 10.1111/cmi.12022
- Issue online: 19 NOV 2012
- Version of Record online: 20 SEP 2012
- Accepted manuscript online: 20 AUG 2012 06:30AM EST
- Manuscript Accepted: 16 AUG 2012
- Manuscript Revised: 3 AUG 2012
- Manuscript Received: 30 MAY 2012
- NIH. Grant Numbers: A1036629, A1084243
- Deutsche Forschungsgemeinschaft. Grant Numbers: SFB635, SFB670
- Washington University
Fig. S1. Wide field epifluorescence imaging of late-stage vacuole localization of ROP18 mutants. Infected HFFs were fixed 24 hpi, permeabilized with digitonin, and processed for indirect immunofluorescence. Samples were stained for a parasite vacuole marker GRA7 with rabbit anti-GRA7 and for ROP18-Ty with mAb BB2 followed by secondary antibodies conjugated to Alexa Fluor 594 and 488 respectively. Scale = 5 μm.
Table S1. Summary of strains used in this study.
Table S2. Summary of primers used in this study.
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