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Fig. S1. Wide field epifluorescence imaging of late-stage vacuole localization of ROP18 mutants. Infected HFFs were fixed 24 hpi, permeabilized with digitonin, and processed for indirect immunofluorescence. Samples were stained for a parasite vacuole marker GRA7 with rabbit anti-GRA7 and for ROP18-Ty with mAb BB2 followed by secondary antibodies conjugated to Alexa Fluor 594 and 488 respectively. Scale = 5 μm.

cmi12022-sup-0001-si.pdf1747K

Table S1. Summary of strains used in this study.

cmi12022-sup-0001-si.pdf1747K

Table S2. Summary of primers used in this study.

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