Equally contributing authors.
Cytosolic herpes simplex virus capsids not only require binding inner tegument protein pUL36 but also pUL37 for active transport prior to secondary envelopment
Version of Record online: 20 DEC 2012
© 2012 Blackwell Publishing Ltd
Special Issue: Scientific Priority Program 1175
Volume 15, Issue 2, pages 248–269, February 2013
How to Cite
Sandbaumhüter, M., Döhner, K., Schipke, J., Binz, A., Pohlmann, A., Sodeik, B. and Bauerfeind, R. (2013), Cytosolic herpes simplex virus capsids not only require binding inner tegument protein pUL36 but also pUL37 for active transport prior to secondary envelopment. Cellular Microbiology, 15: 248–269. doi: 10.1111/cmi.12075
- Issue online: 16 JAN 2013
- Version of Record online: 20 DEC 2012
- Accepted manuscript online: 27 NOV 2012 05:31PM EST
- Manuscript Revised: 19 NOV 2012
- Manuscript Accepted: 19 NOV 2012
- Manuscript Received: 8 OCT 2012
- Deutsche Forschungsgemeinschaft. Grant Numbers: SPP1175, SFB900, TP C2
Movies S1–S4. Vero cells were infected at an MOI of 10 PFU cell−1 with different HSV strains whose capsids had been tagged with CheVP26. Live cell imaging was performed between 8 and 10 h PI using fluorescence confocal microscopy and collecting five images per second for a total time period of 48 s. The mCherry channel was recorded and movie images were inverted. Individual capsids were identified by the intensity of the fluorescence signal and manually tracked. The quantification of the tracks is shown in Fig. 8. The upper panel presents the mCherry channel and the lower panel the mCherry channel overlaid with representative capsid tracks.
Movie S1. Cells infected with HSV1(17+)Lox-CheVP26.
Movie S2. Cells infected with HSV1(17+)Lox-CheVP26-ΔUL36.
Movie S3. Cells infected with HSV1(17+)Lox-CheVP26-ΔUL37.
Movie S4. For the rescue experiment cells were transfected with a plasmid expressing pUL37GFP 2 h prior to infection with HSV1(17+)Lox-CheVP26-ΔUL37.
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