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cmi12075-sup-0001-Movie1.avi3309K

Movies S1–S4. Vero cells were infected at an MOI of 10 PFU cell−1 with different HSV strains whose capsids had been tagged with CheVP26. Live cell imaging was performed between 8 and 10 h PI using fluorescence confocal microscopy and collecting five images per second for a total time period of 48 s. The mCherry channel was recorded and movie images were inverted. Individual capsids were identified by the intensity of the fluorescence signal and manually tracked. The quantification of the tracks is shown in Fig. 8. The upper panel presents the mCherry channel and the lower panel the mCherry channel overlaid with representative capsid tracks.

Movie S1. Cells infected with HSV1(17+)Lox-CheVP26.

cmi12075-sup-0002-Movie2.avi3337K

Movie S2. Cells infected with HSV1(17+)Lox-CheVP26-ΔUL36.

cmi12075-sup-0003-Movie3.avi3435K

Movie S3. Cells infected with HSV1(17+)Lox-CheVP26-ΔUL37.

cmi12075-sup-0004-Movie4.avi3364K

Movie S4. For the rescue experiment cells were transfected with a plasmid expressing pUL37GFP 2 h prior to infection with HSV1(17+)Lox-CheVP26-ΔUL37.

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