Neisseria gonorrhoeae breaches the apical junction of polarized epithelial cells for transmigration by activating EGFR
Version of Record online: 21 JAN 2013
© 2012 John Wiley & Sons Ltd
Volume 15, Issue 6, pages 1042–1057, June 2013
How to Cite
Edwards, V. L., Wang, L.-C., Dawson, V., Stein, D. C. and Song, W. (2013), Neisseria gonorrhoeae breaches the apical junction of polarized epithelial cells for transmigration by activating EGFR. Cellular Microbiology, 15: 1042–1057. doi: 10.1111/cmi.12099
- Issue online: 14 MAY 2013
- Version of Record online: 21 JAN 2013
- Accepted manuscript online: 26 DEC 2012 07:25AM EST
- Manuscript Accepted: 17 DEC 2012
- Manuscript Revised: 13 DEC 2012
- Manuscript Received: 27 JUN 2012
- National Institutes of Health. Grant Number: AI068888
Fig. S1. GC inoculation disrupts the continuous apical junction location of ZO-1 and occludin in polarized T84 cells. Polarized T84 cells were incubated with media only (a) or GC (b–c) in the apical compartment for 6 h. Cells were stained for ZO-1 or occludin and GC and analysed using confocal microscopy. Shown are representative images of ZO-1 (A) and occludin (B) and their fluorescence intensity profiles (d–e). Cells with disrupted ZO-1 (C) and occludin (D) peripheral staining were quantified by visual inspection, and the average percentages (± SD) from three independent experiments are shown. Scale bar, 10 μm. **P ≤ 0.01.
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