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cmi12101-sup-0001-si.pdf3476K

Fig. S1. Characterization of plasma membrane fractions.

A. Western blot analysis of HeLa P4 membranes. Isolation of plasma membranes by the silica bead (left panel) or membrane blebbing (right panel).

B. Lipid composition of MT-4 plasma membranes isolated with silica beads.

Comparison of lipid profiles of plasma membrane purifications using either 1% or 10% silica beads.

Fig. S2. Saturation/unsaturation of fatty acids from glycerophospholipids.

A. Analysis of cell membranes, plasma membranes and HIV-1 particles from HeLa P4 cells. Data represent mean values and SD of n = 3 independent experiments, except for PI in HeLa HIV-1 particles (n = 1).

B. Analysis of cell membranes, plasma membranes and HIV-1 particles from MT-4 cells. Data represent mean values and SD of n = 3 independent experiments.

Fig. S3. Fatty acid chain length distributions in glycerophospholipids.

A. Analysis of cell membranes, plasma membranes and HIV-1 particles from HeLa P4 cells. Data represent mean values and SD of n = 3 independent experiments, except for PI in HeLa HIV-1 particles (n = 1).

B. Analysis of cell membranes, plasma membranes and HIV-1 particles from MT-4 cells. Data represent mean values and SD of n = 3 independent experiments.

Fig. S4. Lipid species distributions within SMs. Analysis of cell membranes, plasma membranes and HIV-1 particles from HeLa P4 and MT-4 cells. Data are displayed as % of total SM species. Data represent mean values and SD of n = 3 independent experiments.

Table S1. Lipid composition of cells, plasma membranes and HIV-1 particles.

cmi12101-sup-0002-si.xlsx88K

Table S2. Lipid composition, species distributions, fatty acid chain length and degree of saturation of cells, plasma membranes and viral particles.

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