Equally contributing authors.
Haemoglobin S and C affect the motion of Maurer's clefts in Plasmodium falciparum-infected erythrocytes
Article first published online: 20 JAN 2013
© 2012 John Wiley & Sons Ltd
Volume 15, Issue 7, pages 1111–1126, July 2013
How to Cite
Kilian, N., Dittmer, M., Cyrklaff, M., Ouermi, D., Bisseye, C., Simpore, J., Frischknecht, F., Sanchez, C. P. and Lanzer, M. (2013), Haemoglobin S and C affect the motion of Maurer's clefts in Plasmodium falciparum-infected erythrocytes. Cellular Microbiology, 15: 1111–1126. doi: 10.1111/cmi.12102
- Issue published online: 13 JUN 2013
- Article first published online: 20 JAN 2013
- Accepted manuscript online: 26 DEC 2012 07:25AM EST
- Manuscript Accepted: 14 DEC 2012
- Manuscript Revised: 30 NOV 2012
- Manuscript Received: 14 SEP 2012
- Deutsche Forschungsgemeinschaft
- Chica and Heinz Schaller foundation
The haemoglobinopathies S and C protect carriers from severe Plasmodium falciparum malaria. We have recently shown that haemoglobin S and C interfere with host-actin remodelling in parasitized erythrocytes and the generation of an actin network that seems to be required for vesicular protein trafficking from the Maurer's clefts (a parasite-derived intermediary protein secretory organelle) to the erythrocyte surface. Here we show that the actin network exerts skeletal functions by anchoring the Maurer's clefts within the erythrocyte cytoplasm. Using a customized tracking tool to investigate the motion of single Maurer's clefts, we found that a functional actin network restrains Brownian motion of this organelle. Maurer's clefts moved significantly faster in wild-type erythrocytes treated with the actin depolymerizing agent cytochalasin D and in erythrocytes containing the haemoglobin variants S and C. Our data support the model of an impaired actin network being an underpinning cause of cellular malfunctioning in parasitized erythrocytes containing haemoglobin S or C, and, possibly, for the protective role of these haemoglobin variants against severe malaria.