Cryptosporidium parvum scavenges LDL-derived cholesterol and micellar cholesterol internalized into enterocytes
Version of Record online: 28 JAN 2013
© 2013 John Wiley & Sons Ltd
Volume 15, Issue 7, pages 1182–1197, July 2013
How to Cite
Ehrenman, K., Wanyiri, J. W., Bhat, N., Ward, H. D. and Coppens, I. (2013), Cryptosporidium parvum scavenges LDL-derived cholesterol and micellar cholesterol internalized into enterocytes. Cellular Microbiology, 15: 1182–1197. doi: 10.1111/cmi.12107
- Issue online: 13 JUN 2013
- Version of Record online: 28 JAN 2013
- Accepted manuscript online: 11 JAN 2013 11:25AM EST
- Manuscript Accepted: 27 DEC 2012
- Manuscript Revised: 13 DEC 2012
- Manuscript Received: 22 OCT 2012
- NIH. Grant Number: AI081562
Fig. S1. Diagrammatic representation of the extracytoplasmic localization of Cryptosporidium at the apex of epithelial cells, showing the feeder organelle consisting of an intravacuolar tubular complex, the desmosome-like band and the accumulation of actin filaments at the PV–host cell junction.
Fig. S2. Fluorescence microscopic observations of extracellular oocysts either untreated (cyst wall intact) or treated with taurocholate and bleach to induce excystation (cyst wall open) after formaldehyde fixation. No fluorescence signal can be detected in any channels. This assay is the control experiment for Fig. 1E for which oocysts has been fixed and exposed to filipin, revealing a UV staining within oocysts upon excystation induction. Arrow shows the progression of sporulation until sporozoite egress. Scale bars are 3 μm.
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