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Fig. S1. Diagrammatic representation of the extracytoplasmic localization of Cryptosporidium at the apex of epithelial cells, showing the feeder organelle consisting of an intravacuolar tubular complex, the desmosome-like band and the accumulation of actin filaments at the PV–host cell junction.

Fig. S2. Fluorescence microscopic observations of extracellular oocysts either untreated (cyst wall intact) or treated with taurocholate and bleach to induce excystation (cyst wall open) after formaldehyde fixation. No fluorescence signal can be detected in any channels. This assay is the control experiment for Fig. 1E for which oocysts has been fixed and exposed to filipin, revealing a UV staining within oocysts upon excystation induction. Arrow shows the progression of sporulation until sporozoite egress. Scale bars are 3 μm.

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