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Fig. S1. Cultured human fully differentiated colon cancer cells. Transmission electron microscopy examination of Caco-2/TC7 cells shows the polarized organization of the cells forming a monolayer (A). Scanning electron microscopy examination of Caco-2/TC7 cells shows the dense and well-organized apical brush border composed of well-ordered microvilli (B to D). In (E) and (F), transmission electron microscopy examination of T84 cells shows the polarized organization of the cells forming a monolayer and the apical brush border composed of short microvilli. The arrows indicates a tight junction (TJ). In (G), scanning electron microscopy examination of HT29-FU cells shows a randomly distributed mucus-secreting cell and adjacent enterocyte-like cells expressing a dense and well-organized brush border. In (H), transmission electron microscopy examination of fully differentiated HT29-MTX cells shows the polarized organization of the cells, the brush border and the intracellular dense vesicles packed with mucins. In (I), confocal laser scanning microscopy examination of SI immunofluorescence labelling in Caco-2/TC7 cells shows the mosaic pattern of expression of the brush border-associated hydrolase (x–z section). In J, confocal laser scanning microscopy examination of TJs-associated ZO-1 protein immunofluorescence labelling shows the expression of the protein at the cell-to-cell contact of confluent T84 cells (x–y section). In (K), scanning electron microscopy examination of HT29-MTX cells shows the dense mucus gel at the cell surface. In (L) and (M), transmission electron microscopy examination of HCT-8 cells shows the cells expressing unorganized cell extensions at the cell periphery. Cells were observed after 15 days (Caco-2/TC7, T84 and HCT-8 cells) and 21 days (HT29-FU and HT29-MTX cells) in culture. T84 micrographs with the courtesy of P. Hofman (Laboratory of Clinical and Experimental Pathology, Pasteur Hospital, CHU Nice, and Medical School, Nice Sophia University, Nice, France). HTCT-8 micrographs with the courtesy of T. Lesuffleur (Inserm, UMRS 938. Research Center Saint-Antoine, Team ‘Instability of Microsatellites and Cancers’, Paris, France).

cmi12126-sup-0002-si.docx35K

Table S1. Characteristics of cultured human fully differentiated colon cancer cell lines, cell subpopulations and clones.

Table S2. Overview of cell interaction, and structural and functional injuries in fully differentiated cancer colon cells.

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