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cmi12136-sup-0001.tif1886K

Fig. S1. Egg chambers (upper panels) and brain sections (lower panels) from wild-caught, Wolbachia-infected D. simulans lines. Host DNA and Wolbachia are stained with Propidium Iodide and Wolbachia are visible as small puncta in the egg chambers or among the host neuroblast nuclei. Scale bars, 20 μM. Similar images were obtained with all examined D. melanogaster lines.

cmi12136-sup-0002.tif2960K

Fig. S2. A. Egg chambers from infected lines (712 and 852), tetracycline-cured lines (712 and 852) and uninfected lines (375 and 517).

B. PCR from infected and cured D. melanogaster lines as indicated. Scale bars, 10 μM.

cmi12136-sup-0003.docx14K

Table S1. Quantification of individual Wolbachia bacteria in different brain regions (data of the summary shown in Figs 3K and 4)

cmi12136-sup-0004.docx14K

Table S2.n of the physiological experiments shown in Fig. 7. Status indications are: I = infected, T = infected, treated, U = uninfected, UT = uninfected, treated.

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