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cmi12156-sup-0001-si.mov9741K

(In all rendered tomograms the membranes are green and the (putative) scaffold in red)

Movie S1. Tomogram and rendering of a VACV factory region. Dual-axis tilt series was generated from 200 nm thick thawed cryo-sections from +60 to −60 degrees and tomogram made in iMOD. The movie corresponds to the slices shown in Fig. 2A and B and the crescent, the small scaffold patch, its associated membranes and a cisternal element close by (blue) were rendered. Bar = 200 nm.

cmi12156-sup-0002-si.avi2225K

Movie S2. Three-dimensional model of the crescent shown in the tomogram in Movie S1 obtained after rendering of Movie S1. The membrane is green and the scaffold is red. Bar = 200 nm.

cmi12156-sup-0003-si.mov3927K

Movie S3. Tomogram of a peripheral area of the Mimivirus factory region. Dual-axis tilt series were generated from 300 nm thick thawed cryo-sections from +60 to −60 degrees. The movie corresponds to the slices shown in Fig. 5A. Bar = 200 nm.

cmi12156-sup-0004-si.avi8070K

Movie S4. Tomogram and rendering of the same area as shown in Movie S3. It shows the membranes in green and the putative capsid in red. The movie shows how the factory-associated membranes are connected to the growing virion whereas the putative scaffold on its convex side is not continuous with other structures. Bar = 200 nm.

cmi12156-sup-0005-si.avi1801K

Movie S5. Three dimensional model of the rendered growing particle in Movie S4. The growing particle is connected to open membranes (green) and shaped by the putative capsid (red) on the convex side. Bar = 100 nm.

cmi12156-sup-0006-si.mov5973K

Movie S6. Tomogram of a peripheral area of the Mimivirus factory region. Dual-axis tilt series were generated from 300 nm thick thawed cryo-sections from +60 to −60 degrees. The movie corresponds to the slices shown in Fig. 5B. Bar = 200 nm.

cmi12156-sup-0007-si.avi7596K

Movie S7. Tomogram and rendering of the same area as shown in Movie S6. It shows the membranes in green and the putative capsid in red. The movie shows how the factory-associated membranes are connected to the growing virion whereas the capsid on its convex side is not continuous with other structures. Bar = 100 nm.

cmi12156-sup-0008-si.avi2658K

Movie S8. Three-dimensional model after rendering of one of the growing particles in Movie S7. 3D model of a growing particle showing the connected open membranes and the capsid on the convex side. Bar = 100 nm.

cmi12156-sup-0009-si.mov4145K

Movie S9. Tomogram of membranes that accumulate at the factory region that are not connected to growing particles. Dual-axis tilt series were generated from 300 nm thick thawed cryo-sections from +60 to −60 degrees. The movie corresponds to the slices shown in Fig. 6A. Bar = 200 nm.

cmi12156-sup-0010-si.avi7263K

Movie S10. Tomogram and rendering of membranes shown in Movie S9. Dual-axis tilt series were generated from 300 nm thick thawed cryo-sections from +60 to −60 degrees. The movie corresponds to the slices shown in Fig. 6. Bar = 100 nm.

cmi12156-sup-0011-si.avi5092K

Movie S11. Rendering of the membranes shown in Movie S9. In three dimensions the VF-associated membranes appear as open membrane sheets connected to form an open-eight conformation. Bar = 100 nm.

cmi12156-sup-0012-si.avi5044K

Movie S12. Tomogram and rendering of control membranes. Tilt series and reconstruction of membranes in Mimivirus-infected cells not located at the periphery of the VF (control membranes) It shows that the green membranes are closed compartments. Bar = 100 nm.

cmi12156-sup-0013-si.avi1101K

Movie S13. Rendering of the Movie S12. The 3D model shows the control membranes as closed compartments. Bar = 100 nm.

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