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cmi12184-sup-0001-si.tif572K

Fig. S1. Phosphorescence of different common catheter types. Three different brands of triple lumen catheters (Arrow, Edwards Swan-Ganz, BBraun Certofix) were cut in 1 cm long pieces and placed in Petri dishes for image acquisition with the BLI camera. Panel A: luminescence image acquired after exposing the catheters to light by opening-closing of the scanner door, showing phosphorescence signal emitted by the triple-lumen Swan-Ganz and Arrow catheter pieces, while no phosphorescence signal was detected for the Certofix catheter pieces. Panel B is the same image as in panel A, showing the overlaid ROIs used for quantification of the background and phosphorescence signals as plotted in Fig. 1A.

cmi12184-sup-0002-si.tif102K

Fig. S2. BLI of yeast-to-hyphal cell ratio. HWPgLuc-expressing C. albicans cells were cultured either to be in the yeast cell from (in YPD medium) or in the hyphal cell form (YP medium containing 10% fetal bovine serum). Candida yeast and/or hyphal cell suspensions of 1 × 107 cells ml−1 were plated in 24-well plates, in a total volume of 1 ml medium, imaged with BLI in triplicate series as follows: HWPgLuc-expressing C. albicans hyphal/yeast cells in ratios (%): 100/0–80/20–60/40–40–60–20/80–0/100. The graph represents the BLI results and linear regression line (R2 = 0.9559), showing near-perfect correlation of the bioluminescence signal intensity with the percentage hyphal content in the sample. Error bars are StDev of triplicate samples.

cmi12184-sup-0003-si.tif487K

Fig. S3. BLI of planktonic bcr1−/−-ACTgLuc cells.

A. BLI of 10-fold dilutions of bcr1−/−-ACTgLuc and SC5314-ACTgLuc (wt control) C. albicans cells, and CTZ alone (n = 3). The BLI-signal emitted from bcr1−/−-ACTgLuc and SC5314-ACTgLuc C. albicans was found to be equal and correlated strongly with the number of cells. (R2 (bcr1−/−-ACTgLuc) = 0.957, R2 (SC5314-ACTgLuc) = 0.999).

B. BLI signal (left graph) and cfu (right graph) quantified from the planktonic cells collected from the lumen of explanted catheters at different time points of biofilm formation by bcr1−/−-ACTgLuc and SC-ACTgLuc C. albicans strains. The means of triplicate samples per catheter (n = 18) were plot. Error bars indicate SD of replicate samples; *P < 0.05, **P < 0.005, ***P < 0.0005).

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