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cmi12185-sup-0001-si.tif1287K

Fig. S1. Confirmation of endogenous tagging of TgNuf2 and TgNdc80 through Western blot analysis. The expected band sizes are indicated below the blot.

cmi12185-sup-0002-si.tif9238K

Fig. S2. Non-deconvolved optical z-sections of Fig. 2F. The sections represent a 2.5 μm stack.

cmi12185-sup-0003-si.tif4024K

Fig. S3. Loss of TgNuf2 results is chromosome missegregation. HFF cells were inoculated with TgNuf2-cKD parasites expressing H2B-YFP and cultured in the presence of ATc for 12 h. Following incubation parasites were methanol-fixed and stained with α-IMC3 (red).

cmi12185-sup-0004-si.tif1176K

Fig. S4. Kinetochore clustering persists in the absence of MORN1. HFF cells were inoculated with MORN1-KO parasites and cultured in the absence (A) or presence (B) of ATc for 36 h. Following incubation parasites were methanol-fixed and stained with α-centrin (red) and α-Nuf2 (green).

cmi12185-sup-0005-si.docx88K

Table S1. List summary of all primer names and sequences (5′ to 3′) mentioned in this study. Restriction enzyme sites are underlined. AttB Gateway recombination sites are in bold font. Ligation independent cloning (LIC) extensions are in bold font and underlined.

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