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cmi12211-sup-0003-si.doc38K

Supporting Experimental Procedures.

cmi12211-sup-0001-si.tif72K

Fig. S1. Effects of SAS cell supernatant after infection with P. gingivalis on cell invasion of Ca9-22 cells. We determined Ca9-22 cell invasion through matrigel-coated transwell membranes at 24 h with and without the supernatant of SAS cells infected with P. gingivalis at an moi of 1. Data are shown as the mean ± SD of three independent experiments.*P < 0.01 (t-test).

cmi12211-sup-0002-si.tif669K

Fig. S2. Effects of NF-kB inhibitor, p38, JNK and ERK1/2-specific inhibitors on cell morphology and proliferation of SAS cells.

A. Light microscopy images showing morphology of SAS cells treated with the inhibitors for 24 h. Control cells were untreated.

B. The proliferation of SAS cells was determined using tetrazolium following treatment with/without the inhibitors for 24 h. Data are expressed as relative to the ratio of treated/untreated (c) and shown as the mean ± SD of three independent experiments. The results were analysed with a t-test.

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