Streptococcal co-infection augments Candida pathogenicity by amplifying the mucosal inflammatory response
Version of Record online: 17 OCT 2013
© 2013 The Authors. Cellular Microbiology published by John Wiley & Sons Ltd.
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Volume 16, Issue 2, pages 214–231, February 2014
How to Cite
Xu, H., Sobue, T., Thompson, A., Xie, Z., Poon, K., Ricker, A., Cervantes, J., Diaz, P. I. and Dongari-Bagtzoglou, A. (2014), Streptococcal co-infection augments Candida pathogenicity by amplifying the mucosal inflammatory response. Cellular Microbiology, 16: 214–231. doi: 10.1111/cmi.12216
The copyright line for this article was changed on 10 March 2015 after original online publication.
- Issue online: 13 JAN 2014
- Version of Record online: 17 OCT 2013
- Accepted manuscript online: 17 SEP 2013 05:30AM EST
- Manuscript Accepted: 10 SEP 2013
- Manuscript Revised: 6 SEP 2013
- Manuscript Received: 18 JUN 2013
- National Institute of Dental and Craniofacial Research. Grant Number: RO1 DE013986
Fig. S1. C. albicans promotes S. oralis colonization in the intestinal tract. DNA in stool samples of mice infected with C. albicans alone (Ca), S. oralis alone (So), or co-infected with C. albicans and S. oralis (CaSo) were analysed by qPCR using primers specific for the S. oralis gtfR gene.
Fig. S2. Mono-infection with S. oralis, at the same total microbial dose as co-infected animals, did not trigger mucosal inflammatory marker gene expression. Pro-inflammatory gene transcripts in tongue tissues of wild type animals on day 5 were assessed by RT-qPCR. Results represent mean fold expression level ± SD compared with uninfected, in 4 animals per group.
Fig. S3. S. oralis but not C. albicans activate pro-inflammatory signalling via TLR2. HEK-Blue™ -hTLR2 cells were challenged with live S. oralis 34 and/or C. albicans overnight and supernatants were analysed for alkaline phosphatase activity. Pam2CSK4 was used as a positive control. Two microbial cell doses were tested (Candida: 104, 105 cells ml−1, S. oralis: 105, 106 cells ml−1). HEK-Blue™ Null1 cells did not respond with alkaline phosphatase secretion to any stimulant (not shown). Error bars represent SD of triplicate experiments, *P < 0.001 compared with medium only.
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