These two authors contributed equally.
The level of H2O2 type oxidative stress regulates virulence of Theileria-transformed leukocytes
Article first published online: 20 OCT 2013
© 2013 The Authors. Cellular Microbiology published by John Wiley & Sons Ltd.
This is an open access article under the terms of the Creative Commons Attribution License, which permits use, distribution and reproduction in any medium, provided the original work is properly cited.
Volume 16, Issue 2, pages 269–279, February 2014
How to Cite
Metheni, M., Echebli, N., Chaussepied, M., Ransy, C., Chéreau, C., Jensen, K., Glass, E., Batteux, F., Bouillaud, F. and Langsley, G. (2014), The level of H2O2 type oxidative stress regulates virulence of Theileria-transformed leukocytes. Cellular Microbiology, 16: 269–279. doi: 10.1111/cmi.12218
- Issue published online: 13 JAN 2014
- Article first published online: 20 OCT 2013
- Accepted manuscript online: 30 SEP 2013 09:25AM EST
- Manuscript Accepted: 20 SEP 2013
- Manuscript Revised: 30 AUG 2013
- Manuscript Received: 15 MAY 2013
- University Paris-Diderot
- Wellcome Trust PhD training fellowship award
- Wellcome Trust post-doctoral fellowship
- Wellcome Trust Special Initiative grant. Grant Number: 075820/A/04/Z
- ANR grant. Grant Number: 11 BSV3 01602
- Inserm, the Cnrs and the Labex ParaFrap. Grant Number: ANR-11-LABX-0024
- BBSRC Institute Strategic Grant
Fig. S1. Infected BL3 lymphocytes display signs of aerobic glycolysis.
A. T. annulata-infected TBL3 cells express HIF-1α to greater levels than non-infected BL3 lymphocytes. Actin amounts were used as a loading control.
B. T. annulata infection strongly induces HK2 expression in TBL3 lymphocytes, whereas as in non-infected BL3 cells HK2 is below the level of detection. Actin was used as a loading control.
C. Non-infected BL3 lymphocytes produce less H2O2 than infected TBL3 lymphocytes.
D. Metabolomic analyses highlighting intracellular metabolites stemming from glycolytic activity associated with hexokinase, pyruvate kinase and lactate dehydrogenase in BL3 compared with TBL3 lymphocytes. TBL3 consume more glucose and produce more lactate than BL3, whereas pyruvate levels remain approximately equivalent. The intracellular lactate levels do not take into account lactate secreted into the culture medium.
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