These authors contributed equally to this work.
Yersinia pseudotuberculosis uses Ail and YadA to circumvent neutrophils by directing Yop translocation during lung infection
Article first published online: 3 NOV 2013
© 2013 John Wiley & Sons Ltd
Volume 16, Issue 2, pages 247–268, February 2014
How to Cite
Paczosa, M. K., Fisher, M. L., Maldonado-Arocho, F. J. and Mecsas, J. (2014), Yersinia pseudotuberculosis uses Ail and YadA to circumvent neutrophils by directing Yop translocation during lung infection. Cellular Microbiology, 16: 247–268. doi: 10.1111/cmi.12219
- Issue published online: 13 JAN 2014
- Article first published online: 3 NOV 2013
- Accepted manuscript online: 9 OCT 2013 08:35PM EST
- Manuscript Accepted: 19 SEP 2013
- Manuscript Revised: 3 SEP 2013
- Manuscript Received: 25 JUL 2013
- Howard Hughes Medical Institute (HHMI)
A Yersinia pseudotuberculosis (Yptb) murine model of lung infection was previously developed using the serotype III IP2666NdeI strain, which robustly colonized lungs but only sporadically disseminated to the spleen and liver. We demonstrate here that a serotype Ib Yptb strain, IP32953, colonizes the lungs at higher levels and disseminates more efficiently to the spleen and liver compared with IP2666NdeI. The role of adhesins was investigated during IP32953 lung infection by constructing isogenic Δail, Δinv, ΔpsaE and ΔyadA mutants. An IP32953ΔailΔyadA mutant initially colonized but failed to persist in the lungs and disseminate to the spleen and liver. Yptb expressing these adhesins selectively bound to and targeted neutrophils for translocation of Yops. This selective targeting was critical for virulence because persistence of the ΔailΔyadA mutant was restored following intranasal infection of neutropenic mice. Furthermore, Ail and YadA prevented killing by complement-mediated mechanisms during dissemination to and/or growth in the spleen and liver, but not in the lungs. Combined, these results demonstratethat Ail and YadA are critical, redundant virulence factors during lung infection, because they thwart neutrophils by directing Yop-translocation specifically into these cells.