Viperin is an iron-sulfur protein that inhibits genome synthesis of tick-borne encephalitis virus via radical SAM domain activity
Article first published online: 3 DEC 2013
© 2013 John Wiley & Sons Ltd
Volume 16, Issue 6, pages 834–848, June 2014
How to Cite
Upadhyay, A. S., Vonderstein, K., Pichlmair, A., Stehling, O., Bennett, K. L., Dobler, G., Guo, J.-T., Superti-Furga, G., Lill, R., Överby, A. K. and Weber, F. (2014), Viperin is an iron-sulfur protein that inhibits genome synthesis of tick-borne encephalitis virus via radical SAM domain activity. Cellular Microbiology, 16: 834–848. doi: 10.1111/cmi.12241
- Issue published online: 16 MAY 2014
- Article first published online: 3 DEC 2013
- Accepted manuscript online: 19 NOV 2013 05:04AM EST
- Manuscript Accepted: 13 NOV 2013
- Manuscript Revised: 26 OCT 2013
- Manuscript Received: 18 APR 2013
- Bundesministerium für Bildung und Forschung (BMBF). Grant Number: 01 KI 0711
- Deutsche Forschungsgemeinschaft (DFG). Grant Numbers: SFB 593, GRK 1216
- Swedish foundation for strategic research. Grant Number: ICA 10-0059
- Swedish research council. Grant Number: 2011-2795
- Kempe Foundation and the laboratory for molecular infection medicine Sweden (MIMS). Grant Number: JCK-1120
- von Behring-Röntgen Stiftung
- LOEWE programme of state Hessen
- Max-Planck Gesellschaft
Fig. S1. FPPS appears not involved in the antiviral activity of viperin against TBEV.
A. Virus RNA from VeroB4 cells treated with different concentrations (0–10 μM) of the FPPS inhibitor Ibandronate at 3 or 6 h before TBEV infection.
B. Effect of Tet-inducible overexpression of FPPS in FLP-IN T Rex cells on viperin. Tet-induced cells were transiently transfected with cDNA constructs expressing viperin or the negative control ΔMx, incubated for 24 h, and then infected with TBEV at an moi of 0.1. FPPS induction was visualized by Western blot analysis (inset). Tet-inducible overexpression of CAT in FLP-IN T Rex cells served as negative control. Mean values and standard deviations from three independent experiments are shown.
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