The flagellum of Trypanosoma brucei is an essential and multifunctional organelle that drives parasite motility and is receiving increased attention as a potential drug target. In the mammalian host, parasite motility is suspected to contribute to infection and disease pathogenesis. However, it has not been possible to test this hypothesis owing to lack of motility mutants that are viable in the bloodstream life cycle stage that infects the mammalian host. We recently identified a bloodstream-form motility mutant in 427-derived T. brucei in which point mutations in the LC1 dynein subunit disrupt propulsive motility but do not affect viability. These mutants have an actively beating flagellum, but cannot translocate. Here we demonstrate that the LC1 point mutant fails to show enhanced cell motility upon increasing viscosity of the surrounding medium, which is a hallmark of wild type T. brucei, thus indicating that motility of the mutant is fundamentally altered compared with wild type cells. We next used the LC1 point mutant to assess the influence of trypanosome motility on infection in mice. Wesurprisingly found that disrupting parasite motility has no discernible effect on T. brucei bloodstream infection. Infection time-course, maximum parasitaemia, number of waves of parasitaemia, clinical features and disease outcome are indistinguishable between motility mutant and control parasites. Our studies provide an important step toward understanding the contribution of parasite motility to infection and a foundation for future investigations of T. brucei interaction with the mammalian host.
African trypanosomes, i.e. Trypanosoma brucei and related species, are protozoan parasites that cause sleeping sickness in humans and related diseases in wild and domestic animals. These parasites cause significant human mortality and limit economic development over vast regions of sub-Saharan Africa. Trypanosome infection of a mammalian host is a multistep process. Following transmission via the bite of an infected tsetse fly, the parasites must first establish and then maintain an infection in the bloodstream despite being extracellular and constantly exposed to the host immune system. Antigenic variation of the parasite surface coat and active clearance of host immunoglobulin bound to the parasite surface enable the trypanosome population to evade immune destruction and persist in the bloodstream indefinitely (Barry and McCulloch, 2001; Borst, 2002; Pays, 2005). Bloodstream infection represents stage one of the disease and manifests clinically as flu-like symptoms with recurrent waves of fever and development of lymphadenopathy (Stich et al., 2002; Rodgers, 2010). After a period of weeks to months, parasites penetrate the blood vessel endothelium and invade the central nervous system (CNS) (Stich et al., 2002; Rodgers, 2010) bringing on stage two of the disease. During this stage, patients develop chronic meningoencephalitis with headaches and neurological changes that severely disturb sleep. Coma and death follow if the disease is left untreated (Stich et al., 2002; Rodgers, 2010).
Trypanosomes are highly motile and the contribution of parasite motility to infection and pathogenesis is a long-standing question. Cell motility in T. brucei is driven by a single flagellum that runs alongside the cell and is laterally connected to the cell body (Ralston et al., 2009). The T. brucei flagellum possesses a canonical ‘9 + 2’ axoneme as the platform for assembly of dynein motors that drive flagellum beating (Fig. S1A–D) (Ralston et al., 2009). The prominent influence of the flagellum on trypanosome biology is apparent even in original descriptions of these organisms, which centred on their distinctive, helical cell locomotion as observed in blood samples from infected amphibians (Gruby, 1843). In those studies the parasite's auger-like movement, twisting and rotating around its long axis as it moved forward, led to the genus name ‘Trypanosoma’, which combines the Greek words trypanon (auger) and soma (body). Since then, parasite motility has captured the attention of many scientists and an ‘undulating membrane’ (now known to correspond to the flagellum) is a prominent feature in most descriptions of these organisms. Early work by Walker (1961) demonstrated that motility is driven by a tractile flagellar beat that initiates at the tip of the flagellum and travels to the flagellum base, the opposite of what is seen in most other eukaryotic flagella. More recent analyses of T. brucei motility using high-speed, high-resolution video microscopy provided adjustments to the original ‘auger-like’ description and further emphasized that parasite motility is a prominent feature of these pathogens in the blood (Rodriguez et al., 2009; Uppaluri et al., 2011; Heddergott et al., 2012; Weisse et al., 2012).
Important recent studies have demonstrated a requirement for trypanosome propulsive motility for infection of the tsetse fly (Rotureau et al., 2013). Parasite motility is also widely considered to be important for infection and pathogenesis in the mammalian host and motility functions of the trypanosome flagellum are receiving increasing attention as potential targets for therapeutic intervention (Broadhead et al., 2006; Ralston and Hill, 2006; Ginger et al., 2008; Ralston et al., 2009). Specific requirements for motility in the mammalian host are not clear, but motility has been hypothesized to participate in parasite immune evasion and for penetration of the blood brain barrier and other extravascular tissues. For example, parasite forward movement has been suggested to drive movement of surface-bound immunoglobulin to the posterior end of the cell, where it is internalized, thereby allowing the parasite to resist opsonization and immune destruction (Engstler et al., 2007). As an extracellular pathogen, T. brucei presumably depends on its own motility for penetration of the vascular endothelium and entry into the CNS. Recent studies have even suggested that T. brucei motility is adapted specifically to the bloodstream environment (Heddergott et al., 2012). Unfortunately, direct investigation of a requirement for parasite motility in any aspect of host infection or pathogenesis has not been possible, because of the lack of viable motility mutants in the bloodstream life cycle stage that infects the mammalian host (Ginger et al., 2008; Ralston and Hill, 2008).
Parasite ‘motility’ refers to propulsive translocation of the cell away from the point of origin, rather than simple writhing of the cell body, which may be caused by uncontrolled flagellum beating. Indeed, all T. brucei motility mutants so far described retain a beating flagellum despite defects in sustained propulsive translocation of the cell (Hutchings et al., 2002; Branche et al., 2006; Broadhead et al., 2006; Ralston and Hill, 2006; 2008). Most T. brucei motility mutants have been generated by RNAi knockdown of specific flagellar proteins and these knockdowns were lethal in bloodstream form cells, leading to the suggestion that disrupting motility is lethal (Branche et al., 2006; Broadhead et al., 2006; Ralston and Hill, 2006; 2008). However, most if not all, T. brucei flagellar protein knockdowns have known or suspected structural defects, including some that are far more substantial than just loss of the targeted protein (Ralston and Hill, 2008). Thus, it is not possible with RNAi alone to distinguish between phenotypes arising from defective motility versus pleotropic consequences of ablating target gene expression.
We recently developed a system to study flagellum protein function that employs loss of function point mutants to replace an endogenous protein, rather than simply depleting proteins via RNAi (Ralston et al., 2011). As such, the system enables one to perturb protein function, while avoiding pleotropic structural defects that might arise when depleting building blocks of axonemal protein complexes. Using this system, we identified a point mutation, K203A/R210A, in the LC1 subunit of outer arm dynein (Fig. S1A–D) that disrupts cell motility, but does not disrupt the structure of outer arm dynein (Ralston et al., 2011). Bloodstream form LC1 K203A/R210A mutants, henceforth referred to as ‘K/R’ mutants, were generated by introducing a Tet-inducible copy of the mutant LC1 gene into a Tet-inducible LC1 knockdown line. Tet induction in the parental LC1 knockdown is lethal, while Tet induction of the K/R mutant yields motility mutants that are viable, thereby offering a unique opportunity to examine the influence of motility on trypanosome infection (Ralston et al., 2011).
In the current work, we analyse parasite motility in multiple environments and demonstrate that motility of the K/R mutant is fundamentally altered compared with wild type cells. The motility defect of the LC1 K/R point mutant is at least as severe as that caused by LC1 knockdown, supporting the idea that lethality in the knockdown is not simply due to defective motility. We also exploit the availability of a viable T. brucei bloodstream-form motility mutant to directly test the requirement for parasite motility in mouse infection and pathogenesis. We find that, contrary to the prevailing notion, establishment and maintenance of infection in the bloodstream, as well as gross pathology and lethal outcome are indistinguishable between wild type and motility mutant parasites.
K/R mutant parasites exhibit similar extent of motility defect as knockdown parasites
The contribution of parasite motility to T. brucei infection and pathogenesis in the mammalian host has not been studied. To address this question, we used the T. brucei K/R motility mutant, a conditional mutant that has normal motility in the absence of tetracycline, but defective motility in the presence of tetracycline (Ralston et al., 2011). As part of the current study, we performed an in-depth analysis of cell motility in the K/R mutant compared with parental LC1 knockdowns to assess the extent of the motility defect. We previously analysed motility in K/R point mutants versus LC1 knockdowns at 24 hours post induction (hpi), but at this time point lethal cell division failure was already evident in the parental line and interfered with motility analysis (Ralston et al., 2011). We therefore examined motility at 12 hpi because at this time point a clear motility defect was evident in the parental line and cell division defects were not yet apparent (Fig. 1). As shown in Fig. 1 and Videos S1, S2 and S4, K/R mutants and LC1 knockdown parasites at 12 hpi both have erratic flagellum beating that does not drive cell propulsion. Knockdown parasites are inviable owing to cell division failure that is evident within 26 hpi (Fig. 1A and D; Video S3), while K/R mutants remain viable. By 72 hpi, the motility defect is even more pronounced in K/R mutants (Fig. 1J and Video S5). In all cases, the flagellum beats rapidly, but the cells lack cell propulsion (Video S5). We also examined flagellar beating using high speed, 1000 frames per second (fps), video microscopy (Video S6). This analysis revealed slower flagellar beating and flagellum tip movements that did not propagate along the cell in knockdown and K/R mutant cells compared with wild type. However, we could not discern a specific beat feature that distinguished LC1 knockdown versus point mutant cells.
The K/R mutant motility defect manifests in the bloodstream environment
The environment encountered by parasites in an animal host differs from that of culture medium and these differences can influence parasite motility. For example, the viscosity of blood is significantly higher than that of culture medium and a hallmark of T. brucei motility is that cell velocity and propulsive motility increase with increasing viscosity of the medium (Heddergott et al., 2012). The ability of the parasite to increase motility when viscosity is raised is a consequence of the distinctive auger-like motility of the organism and is also observed for other microbes, such as spirochaetes, that move via spiral cell motility (Berg and Turner, 1979). As a context for mouse infection experiments, we therefore considered the possibility that increased viscosity might rescue the motility defect of K/R mutants. To test this, we examined parasite motility in normal medium versus medium in which viscosity was increased by adding 0.4% methyl cellulose to approximate the viscosity of blood (Heddergott et al., 2012). In the presence of methyl cellulose, induced K/R cells exhibited a pronounced motility defect relative to uninduced controls (Fig. 2 and Fig. S1E). This defect was evident when assessed by motility traces and total distance travelled over time, i.e. velocity (Fig. 2A and B), but is most clearly evident when assessed by mean squared displacement, which measures propulsive motility, i.e. translocation away from the point of origin (Fig. 2C). Moreover, while addition of methylcellulose increased motility of uninduced cells, it did not significantly increase motility of induced cells. Therefore, rather than being rescued, the motility defect of induced K/R mutants relative to control cells was more pronounced in the presence of methylcellulose.
Another difference in blood versus culture medium is that blood presents a heterogeneous environment, owing to the presence of red blood cells and other cell types. These external obstacles influence microbial cell motility (Berg and Turner, 1979; Heddergott et al., 2012). We therefore examined motility of induced and uninduced K/R parasites in whole blood. The density of red blood cells made it impossible to reliably trace individual trypanosomes using dark field illumination as was done for parasites in culture medium. To overcome this, we generated a K/R mutant cell line that constitutively expresses mCherry fluorescent protein, thereby allowing us to track trypanosome cell movements using fluorescent microscopy. We then examined motility of parasites in whole blood. Both uninduced and induced cells showed increased motility in blood than was observed for parasites in culture medium. Nonetheless, induced K/R cells exhibited a clear motility defect relative to uninduced controls even in whole blood (Fig. 3 and Video S7).
To compare the extent to which cell motility is disrupted in different environments, we determined the mean squared displacement slope for induced relative to uninduced cells in each condition, culture medium with or without methylcellulose, and whole blood (Fig. 3D). The combined data show that the LC1 K/R mutant exhibits a motility defect that is at least as pronounced as that of the parental LC1 knockdown and further, that this defect is manifest in culture medium with or without methyl cellulose, as well as in whole blood (Fig. 3D). Given that this motility defect is penetrant even in whole blood, the K/R mutant presents a unique opportunity for assessing the impact of parasite motility on mouse infection.
Motility mutants establish bloodstream infection and cause trypanosomiasis pathology in mice
K/R mutants that were uninduced or induced for 72 h with tetracycline were used to infect mice as described in Experimental procedures. Following infection, mice were examined and parasitaemia was determined daily. For − Tet controls, parasitaemia was first detected by four days post infection and mice usually developed a single wave of unremitting parasitaemia with terminal outcome by 7–8 days post infection (Fig. 4A and B). One mouse in each group exhibited two waves of parasitaemia, but in no case did infected mice clear the second wave or survive longer than 14 days. These infection parameters mimic what we observed with the parental bloodstream single marker (BSSM) trypanosome cell line (not shown) and are consistent with previous studies using BSSM T. brucei (Emmer et al., 2010). In + Tet mice the infection time-course, maximum parasitaemia, number of waves of parasitaemia and mouse survival times were indistinguishable from − Tet controls (Fig. 4A and B). In the single case where two waves of parasitaemia were observed, the VSG expressed by the infecting population was lost in the second wave (Fig. 4C), indicating that VSG switching remains active in the K/R mutant.
Splenomegaly and anaemia are hallmark clinical features of trypanosomiasis in many hosts including humans (Amole et al., 1982) and we therefore examined infected mice for these pathologies. Mice infected with K/R mutants and treated with tetracycline exhibited weight loss, anaemia and splenomegaly that were indistinguishable from that observed in control (− Tet) infections (Fig. 4D–F). We did not observe any discernible difference in mouse behaviour between − Tet and + Tet infections. Hepatomegaly was not observed in either group of mice (not shown). Therefore, + Tet mice infected with K/R mutants established bloodstream infection and showed clinical features of trypanosomiasis that parallel that observed with − Tet control infections.
Infection of mice by + Tet K/R motility mutants was a surprising result. We considered the possibility that the mouse environment might select for revertants with wild type motility and these wild type parasites are responsible for the infection. We also considered the possibility that Tet was somehow not effective under the conditions used. If either of these situations occurred, parasites from infected + Tet mice would no longer exhibit a motility defect. We therefore examined motility and protein expression in parasites taken directly from infected mice. As shown in Fig. 5, K/R mutants taken directly from + Tet mice retained the motility defect (Fig. 5A and B; Videos 8 and 9) and expression of mutant LC1 protein (Fig. 5C) that were observed in culture. As an independent test, we asked whether Tet treatment was able to clear infection by the parental LC1 knockdown line as well as Tet-inducible trypanin knockdown parasites, both of which are non-viable under + Tet conditions (Ralston et al., 2011). In both cases, Tet treatment cleared the infection within 24 h of treatment and no revertant cells emerged (Fig. 5D and Fig. S1F). Therefore, using multiple independent tests, Tet was effective under the conditions used and mouse infection did not simply select for revertants.
African trypanosomes are deadly pathogens and infection of the mammalian host proceeds via multiple steps. Following a tsetse bite, parasites must first establish infection in the bloodstream and then defend against a potent immune response in order to maintain the infection. Subsequently, parasites invade extravascular tissues, including the CNS, leading to host tissue damage, neurologic dysfunction and an ultimately lethal outcome (Stich et al., 2002; Rodgers, 2010). Here we used trypanosome motility mutants in a mouse infection model to examine impact of parasite motility on the first of these steps, namely establishment and maintenance of a bloodstream infection. We also examined pathogenic features and progression to terminal outcome. Surprisingly, infection dynamics, including time-course, maximum parasitaemia and number of waves of parasitaemia, as well as pathological features and mouse survival rates were indistinguishable between control and motility mutant parasites.
Parasite motility is generally considered to be important for infection and motility functions of the trypanosome flagellum are considered candidate targets for therapeutic intervention (Broadhead et al., 2006; Ralston and Hill, 2006; Ginger et al., 2008). Our studies present the first direct tests of the role for parasite motility in mouse infection and were made possible by availability of viable bloodstream-form motility mutants (Ralston et al., 2011). Previously, T. brucei motility mutants were generated using RNAi to simply deplete endogenous flagellar proteins and this is lethal in cultured bloodstream form cells (Branche et al., 2006; Broadhead et al., 2006; Ralston and Hill, 2006), thereby limiting their utility for mouse infection studies. The K/R mutant differs in that the endogenous LC1 protein is replaced with a non-functional mutant and this mutant is viable (Ralston et al., 2011).
The induced K/R mutant retains some residual motility and we cannot rule out the possibility that this is enough to support any motility needs for bloodstream infection. However, induced K/R mutant parasites exhibit a pronounced motility defect relative to control cells under all conditions examined, i.e. culture medium alone, culture medium containing methyl cellulose to increase viscosity, whole blood and in diluted blood taken directly from infected mice. The defect is at least as severe as that seen in the parental LC1 knockdown line, which is non-viable. Moreover, induced K/R mutants do not show significant increase in velocity or propulsive motility when the viscosity of culture medium is raised. Cell velocity and propulsive motility of control trypanosomes increase when viscosity of the culture medium is raised (Fig. 2) (Heddergott et al., 2012). A hallmark of T. brucei cell propulsion is a spiral motility mechanism (Rodriguez et al., 2009; Heddergott et al., 2012). Specific biophysical features of this type of motion causes microbes that move with a spiral mechanism, such as trypanosomes and spirochaetes, to increase motility when viscosity is increased (Berg and Turner, 1979; Heddergott et al., 2012). This result is the opposite of what happens for organisms such as E. coli that do not use a spiral mechanism (Berg and Turner, 1979; Heddergott et al., 2012). Therefore, our results indicate that more than just being slow, cell movement in induced K/R mutants is fundamentally altered, such that they no longer use a spiral motility mechanism.
We considered the possibility that infection is caused by revertants, which might arise for example if cells become insensitive to tetracycline, become resistant to RNAi, or develop mechanisms to overcome LC1 functional defects. This was not the case, because K/R mutants taken directly from infected mice retain their motility defect. Likewise, revertants did not develop during infection with two independent tet-inducible RNAi lines. Moreover, mice infected with K/R mutants do not show any delay in time-course of infection that would be expected if revertants had to emerge. Our findings therefore demonstrate that T. brucei can accommodate severe disruptions in motility mechanism and overall capacity for cell movement without affecting bloodstream infection. We note that infection via intraperitoneal injection used here differs from the natural route via a tsete bite, and it remains possible that motility may be required for infection through a tsete bite.
While not essential for survival in the bloodstream during acute infection, we favour the idea that parasite motility is required for subsequent steps of infection, such as persistence in the bloodstream during a chronic infection (MacGregor et al., 2011) or penetration of the CNS (Jennings et al., 1979). For example, trypanosome forward motility facilitates removal of host immunoglobulin from the cell surface in culture (Engstler et al., 2007) and this activity likely enables parasite persistence in the bloodstream over extended periods in the face of potent immune responses by the host. Persistent infection also enables CNS invasion (Jennings et al., 1982). It is generally thought that T. brucei requires more than two weeks to gain access to brain tissue (Jennings et al., 1979; Poltera et al., 1980; Gray et al., 1982), although this has been debated recently (Frevert et al., 2012). Rapid progression of infection with 427-derived parasites used in our studies, with maximum mouse survival ≤ 14 days, precludes reliable assessment CNS penetration, and additional work with motility mutants in chronic infection models (Jennings et al., 1979; Gray et al., 1982; MacGregor et al., 2011) will be required. T. brucei isolates that give chronic infection have historically been recalcitrant to molecular genetic manipulation, although advances in this direction have been made recently (Macgregor et al., 2013). 427-derived parasites were selected for our studies because of their experimental tractability and because they allowed the first-ever direct analysis of motility in any aspect of T. brucei infection. The demonstration here that induced K/R mutants are viable and mammalian-infectious will now enable studies of parasite motility in chronic infection models.
Another potential reason to retain motility in the bloodstream may be to keep the parasite prepared for uptake and transmission by the tsetse fly vector. T. brucei development in the tsetse requires an ordered series of parasite movements from the midgut to the salivary glands before the parasites transform into mammalian infectious trypomastigotes (Roditi and Lehane, 2008; Sharma et al., 2009; Rotureau et al., 2012). Indeed, important new studies by Rotureau et al. (2013) report that cell motility is required for parasite development and transmission. In this context, parasite motility might be maintained in bloodstream cells so that the parasite is ready to move through the tsetse following uptake with a blood meal.
The role of parasite motility in infection and pathogenesis of trypanosomiasis is a longstanding question that has not previously been amenable to experimental investigation. As such, development of a motility mutant infection model describe here provides an important foundation for future studies of flagellum biology in the context of host infection.
All animal experiments strictly complied with the Institutional Animal Care and Use Committee of University of California, Los Angeles (Approved protocol permit: ARC # 2001-065).
Cultivation, transfection and RNAi induction of trypanosomes in culture were done as described previously (Ralston et al., 2006). The T. brucei bloodstream trypanin knockdown, LC1 knockdown, LC1-K203A/R210A (K/R) motility mutant and BSSM, cells were described previously (Wirtz et al., 1999; Ralston et al., 2011). K/R-mCherry cells were generated by stable transfection of K/R cells with the pNKmCherry plasmid (described below). To generate pNKmCherry, the mCherry gene (accession number AY678264) was PCR amplified from pmCherry plasmid (Hajagos et al., 2012) and cloned into the expression vector pHD496-H (Hutchings et al., 2002) using the HindIII and BamHI cloning sites. pHD496-H contains a ribosomal RNA promoter to drive constitutive expression of the mCherry gene and a hygromycin-resistance cassette to enable selection (Biebinger and Clayton, 1996; Hutchings et al., 2002). Primers used to PCR amplify mCherry are as follow: 5′-ATGGTGAGCAAGGGCGAGG-3′ (forward primer), 5′-TTACTTGTACAGCTCGTCCATGC-3′ (reverse primer). All DNA sequences were verified by direct sequencing.
Mouse infections were done essentially as described (Dubois et al., 2005). BALB/c female mice (The Jackson Laboratory, Bar Harbor, ME), 6–10 weeks old, were injected intraperitoneally with 100 parasites in 0.2 ml cold phosphate-buffered saline (PBS) (pH 7.4) supplemented with 1% glucose. To maintain cell viability in PBS, parasites were kept on ice prior to injection into mice. For + Tet infections, parasites were induced with 1 μg ml−1 tetracycline in culture three days prior to injection into mice and mice received 1 mg ml−1 doxycycline, a tetracycline version, in their drinking water 5–7 days prior to infection and throughout the course of infection. Parasitaemia was monitored daily beginning 3 days post infection using an improved Neubaeur hemocytometer (Herbert and Lumsden, 1976). After euthanasia, mice were weighed and tissues were collected for weighing. For trypanin knockdown and LC1 knockdown infections, parasites were maintained in culture without tetracycline and mice received 1 mg ml−1 doxycycline in drinking water only when animals showed the first wave of parasitaemia.
Parasite motility assays
Knockdown, K/R and K/R-mCherry parasites were grown without or with tetracycline to a density of 1.5–2.0 × 106 cells ml−1 and cell motility analysis was done at the hours post induction (hpi) described in each figure. For methyl cellulose experiments, cells were maintained with or without addition of 0.4% (v/v) to approximate the viscosity of blood (Heddergott et al., 2012). For mCherry K/R parasites, cells (50 μl) were added to freshly drawn, undiluted mouse blood (500 μl). All samples were analysed in a pre-warmed motility chambers within 5 min after collection. Motility traces were done as described (Ralston et al., 2011). Briefly, parasites were examined in motility chambers using dark field optics on a Zeiss Axioskop II compound microscope with a 10× objective (Figs 1 and 2) or using fluorescence optics on a Zeiss Axiovert 200 M inverted microscope with a 20× objective (Fig. 3). Videos were captured using a COHU analogue video camera. Analogue format movies were converted to digital format with an ADVC-300 digital video converter (Canopus). Movies were recorded at 30 fps and converted to AVI format and then to stacks of TIFF images using Adobe Premiere Elements software (Adobe Systems). TIFF image stacks were analysed using Metamorph software (Molecular Devices) to trace parasite movement over the indicated time period. Trace data were used to calculate the total distance travelled, as well as the mean squared displacement (MSD) of individual cells in the x and y dimensions. MSD was calculated according to the formula MSD = < ri(t)2 > = < [pi(t) − pi(0)]2 >, where ri is the distance travelled by the parasite over time interval t and pi is the position of the parasite at any given time. For Figs 1 and 2, the timescale of t ranged from 1 to 25 s in increments of 1 s. For Figs 3 and 5, the timescale of t ranged from 1 to 8 seconds in increments of 0.3 second. MSD is calculated for each instance i of a given time interval. Several cell MSDs were then averaged to obtain an ensemble average.
For motility experiments on parasites from mouse infections (Fig. 5), blood samples were collected from infected mice 7–13 days post infection (dpi). Samples were kept at 37°C and analysed in a pre-warmed motility chamber within 5–10 min after collection. To record motility traces, blood samples were diluted 1:100 in warm HMI-9 culture medium to enable tracing of individual parasites among red blood cells and videos were recorded and processed as described above, using differential interference contrast (DIC) optics on a Zeiss Axiovert 200 M inverted microscope with a 100× oil-immersion objective. For high-resolution videos of individual cells (Videos S1–9), videos were captured in motility chambers using DIC optics (Videos S1–6 and S8–9) or using fluorescence optics (Video S7) on a Zeiss Axiovert 200 M inverted microscope with a 100× oil-immersion (Videos S1–6 and S8–9) or 20× (Video S7) objective.
Hi-speed videos: The experimental setup used to record high-speed trypanosome motility consists of an inverted DMI6000 B microscope (Leica Microsystems, Wetzlar, Germany) equipped with a 100× objective (Leica HCX PL APO 100×/1.40-0.70 oil) and DIC optics, a halogen-based white trans-illumination system and a PC-controlled Photron FocusScope SV200-i high-speed CMOS camera (Photron USA) mounted in the trinocular port. The images provided by the high-speed camera are directly observed in the PC monitor. System control is achieved through the Photron FASTCAM Viewer software (PFV Version 3). Images were captured at 1000 fps with 512 × 512 pixel image resolution.
Western blot analysis of parasites from mice
Western blots were done as described previously (Ralston et al., 2011). To obtain parasites from mice, mice were euthanized and blood was collected in heparinized tubes 7–13 days post infection. Parasites were separated from whole blood using DE 52 anion exchange columns, as described (Lanham and Godfrey, 1970). Purified parasites were examined with a microscope to assess purity and parasite yield was determined by counting in a hemocytometer. Purified parasites were washed three times in PBS and then boiled in Laemmli sample buffer for Western blot analysis. Blots were probed with anti-HA monoclonal antibody (Covance) at 1:5000 dilution, anti-β-tubulin monoclonal antibody (Developmental Studies Hybridoma Bank, University of Iowa) at 1:5000 dilution, or anti-variable surface glycoprotein VSG 221 (McDowell et al., 1998) at 1:100 000 dilution.
Statistical analysis and graphical output was done with GraphPad Prism 5. The significance of survival was determined by Log-rank (Mantel-Cox) Test. We used the Student unpaired two-tailed t-test to determine significant differences in motility. The significance of total body weight, spleen weight, liver weight, and number of red blood cells was calculated by one-way anova with Bonferroni's post-test for multiple comparisons. Significance (P-value) is reported in each figure.
Funding for this work was supplied by grants to K.L.H. from the Burroughs Wellcome Fund and NIH-NIAID (AI052348). Neville K. Kisalu is a recipient of the Shapiro Fellowship and the UCLA Dissertation Year Fellowship. Gerasimos Langousis is recipient of the Warsaw fellowship. We are grateful to John Mansfield and Karen Demick (University of Wisconsin) for guidance with mouse infections. We thank Jose Rodriguez (UCLA) for technical support with motility trace analysis. We thank Josh Beck (UCLA) for kindly providing us with the mCherry DNA. We are also appreciative of Shimon Weiss (UCLA) and members of our laboratory for pertinent comments on the work and critical reading of the manuscript. High-speed video microscopy was performed at the California NanoSystems Institute Advanced Light Microscopy/Spectroscopy and the Macro-Scale Imaging Shared Facilities at UCLA. Authors have no conflict of interest.
Conceived the study: N.K.K, K.S.R, K.L.H. Performed the experiments: N.K.K, G.L, L.A.B. Analysed the data: N.K.K, G.L, K.S.R, L.A.B, K.L.H. Wrote the paper: N.K.K and K.L.H.