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cmi12244-sup-0001-fig_s1.tif741K

Fig. S1. A. Cartoon of a T. brucei cell.

B. A schematic diagram of an axoneme cross section as would be observed looking posterior to anterior near the boxed region in (A).

C. Simplified diagram of outer arm dynein, showing the two heavy chains (α and β) and the location of LC1.

D. LC1 schematic showing residues mutated in the K/R mutant used in this study. Panel A adapted from (Hill et al., 2000) with permission.

E. Mean squared displacement of K/R parasites in media without or with methyl cellulose. Same graph as shown in Fig. 2C, but including error bars that show standard error of the mean (SEM). See Fig. 2C legend for details.

F. Tetracycline treatment clears infection by trypanin knockdown parasites. Parasitaemias of two mice infected with trypanin knockdown parasites (TPN-KD). Mice were infected with TPN-KD parasites at day 0 and then treated with tetracycline in drinking water upon detection of parasites in blood (+ Tet arrows). Mice cleared infection within 24 h of tetracycline treatment, demonstrating the efficacy of tetracycline treatment.

cmi12244-sup-0002-video_s1.mov1548KVideo S1. Wild type motility of LC1 knockdown trypanosomes in − Tet cultures. Representative live video shows propulsive motility of LC1 knockdown parasites taken from − Tet cultures. Parasites move rapidly and translocate with tip of flagellum leading. Frame rate for capture and playback is 30 frames s−1.
cmi12244-sup-0003-video_s2.mov1912KVideo S2. Defective motility of LC1 knockdown trypanosomes from + Tet cultures (12 hpi). Representative live video shows defective motility of LC1 knockdown parasites grown in + Tet cultures for 12 h. Flagellum beating is evident, but parasite propulsive motility is disrupted. Frame rate for capture and playback is 30 frames s−1.
cmi12244-sup-0004-video_s3.mov1822KVideo S3. Defective motility by LC1 knockdown trypanosomes from + Tet cultures (26 hpi). Representative live video shows cell division failure of LC1 knockdown parasites grown in + Tet cultures for 26 h. Cells accumulate as an amorphous mass, but the flagella continue to beat vigorously. Frame rate for capture and playback is 30 frames s−1.
cmi12244-sup-0005-video_s4.mov2351KVideo S4. Defective motility by K/R trypanosomes from + Tet cultures (12 hpi). Representative live video shows defective motility of K/R parasites grown in + Tet cultures for 12 h. Flagellum beating is evident, but parasite propulsive motility is disrupted. Frame rate for capture and playback is 30 frames s−1.
cmi12244-sup-0006-video_s5.mov2202KVideo S5. Defective motility by K/R trypanosomes from + Tet cultures (72 hpi). Representative live video shows defective motility of K/R parasites grown in + Tet cultures for 72 h. Flagellum beating is evident, but parasite propulsive motility is disrupted. Frame rate for capture and playback is 30 frames s−1.
cmi12244-sup-0007-video_s6.mov4608KVideo S6. Combined high-speed videos of flagellar beating in minus Tet (control) trypanosomes, LC1 Knockdown and K/R trypanosomes from + Tet cultures. The first clip (from 0 to 8 s) shows representative flagellar beating in control cells (LC1 grown in the absence of Tet). Tip-to-base flagellar beats are evident. The second clip (from 10 to 18 s) shows representative flagellar beating in LC1 knockdown parasites grown in + Tet cultures for 12 h. The third clip (from 20 to 28 s) shows representative flagellar beating in K/R parasites grown in + Tet cultures for 12 h. The fourth clip (from 31 to 39 s) shows representative flagellar beating in K/R parasites grown in + Tet cultures for 72 h. For all videos, frame rate for capture is 1000 frames s−1 and playback is 60 frames s−1.
cmi12244-sup-0008-video_s7.mov423KVideo S7. Combined videos show wild type motility of mCherry K/R trypanosomes from − Tet cultures and defective motility of mCherry K/R trypanosomes from + Tet cultures (72 hpi) in whole blood. The first clip (from 0 to 5 s) shows representative motility of mCherry K/R trypanosomes from − Tet cultures, added to mouse blood. Parasites move rapidly and translocate with tip of flagellum leading. Frame rate for capture and playback is 30 frames s−1. The second clip (from 7 to 12 s) shows representative motility of mCherry K/R trypanosomes from + Tet cultures, added to mouse blood. Flagellum beating is evident, but parasite propulsive motility is disrupted. Frame rate for capture and playback is 30 frames s−1. For both videos, cultured trypanosomes were added directly to whole blood.
cmi12244-sup-0009-video_s8.mov2643KVideo S8. Wild type motility of K/R trypanosomes from − Tet mouse infections. Representative live video shows propulsive motility of parasites taken from mice infected with K/R trypanosomes and maintained without tetracycline. Parasites move rapidly and translocate with tip of flagellum leading. Samples were taken 7 days post infection. Red blood cells are readily distinguishable from parasites as biconcave disks. Frame rate for capture and playback is 30 frames s−1.
cmi12244-sup-0010-video_s9.mov2362KVideo S9. Defective motility of K/R trypanosomes from + Tet mouse infections. Representative live video shows defective motility of parasites taken from mice infected with K/R trypanosomes and maintained with tetracycline in the drinking water. Flagellum beating is evident, but parasite propulsive motility is blocked. Samples were taken 7 days post infection. Red blood cells are readily distinguishable from parasites as biconcave disks. Frame rate for capture and playback is 30 frames s−1.

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