SEARCH

SEARCH BY CITATION

FilenameFormatSizeDescription
cmi12257-sup-0001-si.tif4990K

Fig. S1. Phylogenetic analysis of Saa protein sequences.

A. Maximum likelihood phylogenetic tree of human (Homo sapiens, Hs) SAA1 (NP_000322), SAA2 (NP_110381) and SAA4 (NP_006503); mouse (Mus musculus, Mm) Saa1 (NP_033143), Saa2 (NP_035444), Saa3 (NP_035445) and Saa4 (NP_035446); chicken (Gallus gallus, Gg) Saa (ADF56353); rainbow trout (Oncorhynchus mykiss, Om) Saa (NP_001117908); Tetraodon (Tetraodon nigroviridis, Tn) Saa (CAF99678); amphioxus (Branchiostoma belcheri, Bb) Saa (BAB97379); and zebrafish (Danio rerio, Dr) Saa (NP_001005599) and SaaL1 (NP_956429). Zebrafish SaaL1 is included as an outgroup. Bootstrap support (≥ 50%) is shown as results from 1000 bootstrap replicates.

B. Multiple sequence alignment of Saa proteins. Identical residues are highlighted in black, and similar residues are highlighted in grey.

cmi12257-sup-0002-si.tif6793K

Fig. S2. PCR validation of saa MO knockdown. Injection of 1-cell stage embryos with 9 pmol saa.i2e3 morpholino (saa MO) results in ∼150 bp reduction in a subset of saa transcripts in both GF and CONVD larvae at 6dpf. This is consistent with saa.i2e3 MO causing exclusion of exon 3 (154 bp) from saa transcripts. These saa MO-induced splicing defects were not observed following injection of a standard control morpholino (Ctrl MO). Data from two biological replicate pools (#1 and #2; 5–15 larvae per pool) are shown. Note that the total saa transcript levels are increased in CONVD compared with GF controls, but that the magnitude of saa transcript increase varies between replicates.

cmi12257-sup-0003-si.mov6823K

Movie S1. Neutrophil migration in GF zebrafish larvae. Live 6dpf Tg(mpx:GFP) GF zebrafish imaged at a rate of one frame every 15 s for 15 min reveals decreased neutrophil migration compared with CONVD controls (compared with Movie S2).

cmi12257-sup-0004-si.mov6991K

Movie S2. Neutrophil migration in CONVD zebrafish larvae. Live 6dpf Tg(mpx:GFP) CONVD zebrafish imaged at a rate of one frame every 15 s for 15 min reveals increased neutrophil migration compared with GF controls (compared with Movie S1).

cmi12257-sup-0005-si.mov1728K

Movie S3. Neutrophil migration in GF Ctrl-MO zebrafish larvae. Live 6dpf Tg(mpx:GFP) GF zebrafish injected with standard control MO (Ctrl MO) were imaged at a rate of one frame every 15 s for 5 min (compared with Movies S4–S6).

cmi12257-sup-0006-si.mov1574K

Movie S4. Neutrophil migration in CONVD Ctrl-MO zebrafish larvae. Live 6dpf Tg(mpx:GFP) CONVD zebrafish injected with standard control MO (Ctrl MO) were imaged at a rate of one frame every 15 s for 5 min (compared with Movies S3, S5 and S6).

cmi12257-sup-0007-si.mov1662K

Movie S5. Neutrophil migration in GF saa-MO zebrafish larvae. Live 6dpf Tg(mpx:GFP) GF zebrafish injected with saa MO were imaged at a rate of one frame every 15 s for 5 min (compared with Movies S3, S4 and S6).

cmi12257-sup-0008-si.mov1547K

Movie S6. Neutrophil migration in CONVD saa-MO zebrafish larvae. Live 6dpf Tg(mpx:GFP) CONVD zebrafish injected with saa MO were imaged at a rate of one frame every 15 s for 5 min (compared with Movies S3–S5).

Please note: Wiley Blackwell is not responsible for the content or functionality of any supporting information supplied by the authors. Any queries (other than missing content) should be directed to the corresponding author for the article.