Fig. S1. Stx2 is the main toxin type released during infection with EDL933. Undiluted apical supernatants (SN ud) or supernatants diluted 1:10 (SN 1:10) from EDL933-infected T84 cells were incubated with anti-Stx1, anti-Stx2 or medium control (no Ab) before Vero cells were added. Vero cell cytotoxicity is evident in SN samples without antibodies and with anti-Stx1 but not with anti-Stx2.


Fig. S2. Correlation of Stx2 concentrations determined by ELISA and VCCA. Polarized T84 cells were infected for 5 h under AE (■) or MA (□) conditions. Stx2 levels in apical supernatants were quantified by sandwich ELISA or VCCA. Data are shown as means ± SEM from three independent experiments performed in duplicate.

Please note: Wiley Blackwell is not responsible for the content or functionality of any supporting information supplied by the authors. Any queries (other than missing content) should be directed to the corresponding author for the article.