Shiga toxin production and translocation during microaerobic human colonic infection with Shiga toxin-producing E. coli O157:H7 and O104:H4
Article first published online: 21 MAR 2014
© 2014 The Authors. Cellular Microbiology published by John Wiley & Sons Ltd.
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Volume 16, Issue 8, pages 1255–1266, August 2014
How to Cite
Tran, S.-L., Billoud, L., Lewis, S. B., Phillips, A. D. and Schüller, S. (2014), Shiga toxin production and translocation during microaerobic human colonic infection with Shiga toxin-producing E. coli O157:H7 and O104:H4. Cellular Microbiology, 16: 1255–1266. doi: 10.1111/cmi.12281
- Issue published online: 26 JUL 2014
- Article first published online: 21 MAR 2014
- Accepted manuscript online: 26 FEB 2014 02:21AM EST
- Manuscript Accepted: 14 FEB 2014
- Manuscript Revised: 31 JAN 2014
- Manuscript Received: 19 SEP 2013
- Medical Research Council. Grant Number: MR/J002062/1
- Wellcome Trust. Grant Number: WT082560MA
Fig. S1. Stx2 is the main toxin type released during infection with EDL933. Undiluted apical supernatants (SN ud) or supernatants diluted 1:10 (SN 1:10) from EDL933-infected T84 cells were incubated with anti-Stx1, anti-Stx2 or medium control (no Ab) before Vero cells were added. Vero cell cytotoxicity is evident in SN samples without antibodies and with anti-Stx1 but not with anti-Stx2.
Fig. S2. Correlation of Stx2 concentrations determined by ELISA and VCCA. Polarized T84 cells were infected for 5 h under AE (■) or MA (□) conditions. Stx2 levels in apical supernatants were quantified by sandwich ELISA or VCCA. Data are shown as means ± SEM from three independent experiments performed in duplicate.
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