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Fig. S1. Northern analysis of various FmqD::GFP strains. FmqD::GFP mRNA levels are much higher in both the OEFmqD::GFP and OEFmqD[Δ1–21]::GFP strains compared to that of FmqD::GFP and FmqD[Δ1–21]::GFP where the fusion proteins are placed under the governance of the native fmqD promoter. Top row represents fmqD transcript while bottom row represents a gel image of the ribosomal RNAs.

Fig. S2. Time-course fluorescence microscopy of FmqD localization during spore germination. First three panels show FmqD localization in spores at 2 h post inoculation (HPI) of WT, OEFmqD[Δ1–21]::GFP, and OEFmqD::GFP respectively. Panels 3–6 and 7–9 depict FmqD localization in spores at 6 and 10 HPIs respectively. Top row represents GFP images of spores while bottom row represents the merged GFP and differential interference contrast (DIC) images of spores.

Fig. S3. OEFmqA::GFP does not colocalize with endocytic membrane and nuclear stains, FM4–64 Hoescht respectively. Top row: OEFmqA::GFP stained with FM4–64. Bottom row: OEFmqA::GFP stained with Hoescht. From left to right: Merged imaged of GFP and FM4–64/Hoechst 33342, GFP alone, FM4–64/Hoechst 33342 alone, and differential interference contrast (DIC) alone.

Fig. S4. Southern analyses of Fq mutants.

Fig. S5. LC-MS analysis of the methanol extract of an agar core prepared from Af293 WT. TIC, total ion current chromatogram; DAD, diode-array detector (UV254nm trace); EIC, extracted ion chromatogram.

Fig. S6. LC-MS analysis of the methanol extract of an agar core prepared from ΔfmqA. TIC, total ion current chromatogram; DAD, diode-array detector (UV254nm trace); EIC, extracted ion chromatogram.

Fig. S7. LC-MS analysis of the methanol extract of an agar core prepared from ΔfmqB. TIC, total ion current chromatogram; DAD, diode-array detector (UV254nm trace); EIC, extracted ion chromatogram.

Fig. S8. LC-MS analysis of the methanol extract of an agar core prepared from ΔfmqC. TIC, total ion current chromatogram; DAD, diode-array detector (UV254nm trace); EIC, extracted ion chromatogram.

Fig. S9. LC-MS analysis of the methanol extract of an agar core prepared from ΔfmqD. TIC, total ion current chromatogram; DAD, diode-array detector (UV254nm trace); EIC, extracted ion chromatogram.

Table S1. Primers used in this study.

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