Ximei Huang and Xue Yan Yam contributed equally to this work.
Identification of a new export signal in Plasmodium yoelii: identification of a new exportome
Article first published online: 4 APR 2014
© 2014 John Wiley & Sons Ltd
Special Issue: Malaria
Volume 16, Issue 5, pages 673–686, May 2014
How to Cite
Siau, A., Huang, X., Yam, X. Y., Bob, N. S., Sun, H., Rajapakse, J. C., Renia, L. and Preiser, P. R. (2014), Identification of a new export signal in Plasmodium yoelii: identification of a new exportome. Cellular Microbiology, 16: 673–686. doi: 10.1111/cmi.12293
- Issue published online: 15 APR 2014
- Article first published online: 4 APR 2014
- Accepted manuscript online: 18 MAR 2014 01:33AM EST
- Manuscript Revised: 7 MAR 2014
- Manuscript Accepted: 7 MAR 2014
- Manuscript Received: 22 JAN 2014
- Biomedical Research Council – Singapore Immunology Network. Grant Number: BMRC SIGN-07-009
Development of the erythrocytic malaria parasite requires targeting of parasite proteins into multiple compartments located within and beyond the parasite confine. Beyond the PEXEL/VTS pathway and its characterized players, increasing amount of evidence has highlighted the existence of proteins exported using alternative export-signal(s)/pathway(s); hence, the exportomes currently predicted are incomplete. The nature of these exported proteins which could have a prominent role in most of the Plasmodium species remains elusive. Using P. yoelii variant proteins, we identified a signal associated to lipophilic region that mediates export of P. yoelii proteins. This non-PEXEL signal termed PLASMED is defined by semi-conserved residues and possibly a secondary structure. In vivo characterization of exported-proteins indicated that PLASMED is a bona fide export-signal that allowed us to identify an unseen P. yoelii exportome. The repertoire of the newly predicted exported proteins opens up perspectives for unravelling the remodelling of the host-cell by the parasite, against which new therapies could be elaborated.