Deceased on 31 December 2012.
Characterization of antiviral and antibacterial activity of Bombyx mori seroin proteins
Article first published online: 2 APR 2014
© 2014 John Wiley & Sons Ltd
Volume 16, Issue 9, pages 1354–1365, September 2014
How to Cite
Singh, C. P., Vaishna, R. L., Kakkar, A., Arunkumar, K. P. and Nagaraju, J. (2014), Characterization of antiviral and antibacterial activity of Bombyx mori seroin proteins. Cellular Microbiology, 16: 1354–1365. doi: 10.1111/cmi.12294
- Issue published online: 25 AUG 2014
- Article first published online: 2 APR 2014
- Accepted manuscript online: 17 MAR 2014 01:24AM EST
- Manuscript Accepted: 7 MAR 2014
- Manuscript Revised: 24 FEB 2014
- Manuscript Received: 1 OCT 2013
- Centre of Excellence on Genetics and Genomics of Silkmoths. Grant Number: BT/01/COE/05/12
- Department of Biotechnology, Government of India
Table S1. Sequences of primers used in this study.
Fig. S1. B. mori larvae phenotype analysis upon knockdown of seroins, 4 days post dsRNA administration. Knockdown of both seroins resulted in stunted growth as well as marked difference in the appearance of larvae, in uninfected (A) and in BmNPV infected larvae (B).
Fig. S2. RT-PCR based expression analysis of seroins in different tissues of B. mori. 18S rRNA was used as an endogenous control.
Fig. S3. BmNPV infectivity reduced upon BmNPV's budded virion incubation with seroin proteins.
A. BmNPV load determined by estimating amount of viral DNA by qPCR using ie1 specific primers, a BmNPV early gene.
B. Viral OB numbers significantly reduced upon incubation of BVs prior to infection. For qPCR analysis, three independent experiments were carried out in three replicates each with a set of 3 larvae, and the results were normalized against endogenous 18S rRNA. Data are presented as Mean ± SD (n = 3).
Fig. S4. Integrity analysis of recombinant Seroin1 and Seroin2 proteins after purification, performed on 15% SDS-PAGE.
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