Anaplasma phagocytophilum surface protein AipA mediates invasion of mammalian host cells (pages 1133–1145)
David Seidman, Nore Ojogun, Naomi J. Walker, Juliana Mastronunzio, Amandeep Kahlon, Kathryn S. Hebert, Sophia Karandashova, Daniel P. Miller, Brittney K. Tegels, Richard T. Marconi, Erol Fikrig, Dori L. Borjesson and Jason A. Carlyon
Article first published online: 3 APR 2014 | DOI: 10.1111/cmi.12286
Anaplasma phagocytophilum is an obligate intracellular bacterium that utilizes multiple surface proteins called invasins that act cooperatively to mediate entry into host cells. Blocking infection therefore requires simultaneous targeting of the relevant invasins. Herein, we identify a novel A. phagocytophilum invasin, AipA, delineate its invasion domain, and demonstrate that antibodies targeting AipA together with two additional invasins – OmpA and Asp14 – block infection of host cells. Our work evidences the potential of multi-target approaches for preventing infections by intracellular bacteria.