Conflicts of interest: Magnus Bruze is a member of the expert panel of the Research Institute for Fragrance Materials (RIFM), which is supported by the manufacturers of fragrances and consumer products containing fragrances. Martin Mowitz, Cecilia Svedman and Erik Zimerson declare no conflict of interest. Funding sources: None.
Patch testing with serial dilutions and thin-layer chromatograms of oak moss absolutes containing high and low levels of atranol and chloroatranol
Version of Record online: 19 SEP 2013
© 2013 John Wiley & Sons A/S. Published by John Wiley & Sons Ltd
Volume 69, Issue 6, pages 342–349, December 2013
How to Cite
Mowitz, M., Zimerson, E., Svedman, C. and Bruze, M. (2013), Patch testing with serial dilutions and thin-layer chromatograms of oak moss absolutes containing high and low levels of atranol and chloroatranol. Contact Dermatitis, 69: 342–349. doi: 10.1111/cod.12126
- Issue online: 12 NOV 2013
- Version of Record online: 19 SEP 2013
- Manuscript Accepted: 27 JUN 2013
- Manuscript Revised: 4 JUN 2013
- Manuscript Received: 28 MAR 2013
- Evernia prunastri extract;
- oak moss absolute
Oak moss absolute (Evernia prunastri extract) contains a large number of substances, among them the potent allergens atranol and chloroatranol. Since 2008, their content in oak moss absolute has been restricted by the International Fragrance Association to a maximum level of 100 ppm each.
To compare the elicitation capacities of a traditional (sample A) and a treated (sample B) oak moss absolute containing, in total, 27 000 and 66 ppm of atranol and chloroatranol, respectively, and to investigate reactions to components of oak moss absolute separated by thin-layer chromatography (TLC).
Fifteen oak moss-allergic subjects were patch tested with serial dilutions and TLC strips of samples A and B.
Fifteen subjects reacted to sample A at concentrations ≤ 2.0%, and 2 subjects reacted to sample B at 2.0% but not to lower concentrations. Among 13 subjects reacting to the TLC strip of sample A, 11 reacted to spots with retardation factor values corresponding to those of atranol and/or chloroatranol, and 11 reacted to other areas on the TLC strip. Only one subject reacted to the TLC strip of sample B.
The patch test reactivity of sample B was significantly lower than that of sample A. The TLC patch tests indicate the presence of sensitizers other than atranol and chloroatranol in oak moss absolute.