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Patch testing with serial dilutions and thin-layer chromatograms of oak moss absolutes containing high and low levels of atranol and chloroatranol

Authors

  • Martin Mowitz,

    Corresponding author
    1. Department of Occupational and Environmental Dermatology, Skåne University Hospital, Lund University, Malmö, Sweden
    • Correspondence: Martin Mowitz, Department of Occupational and Environmental Dermatology, Skåne University Hospital, 205 02 Malmö, Sweden. Tel: +46 40337410; Fax: +46 40336213. E-mail: martin.mowitz@med.lu.se

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  • Erik Zimerson,

    1. Department of Occupational and Environmental Dermatology, Skåne University Hospital, Lund University, Malmö, Sweden
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  • Cecilia Svedman,

    1. Department of Occupational and Environmental Dermatology, Skåne University Hospital, Lund University, Malmö, Sweden
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  • Magnus Bruze

    1. Department of Occupational and Environmental Dermatology, Skåne University Hospital, Lund University, Malmö, Sweden
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  • Conflicts of interest: Magnus Bruze is a member of the expert panel of the Research Institute for Fragrance Materials (RIFM), which is supported by the manufacturers of fragrances and consumer products containing fragrances. Martin Mowitz, Cecilia Svedman and Erik Zimerson declare no conflict of interest. Funding sources: None.

Summary

Background

Oak moss absolute (Evernia prunastri extract) contains a large number of substances, among them the potent allergens atranol and chloroatranol. Since 2008, their content in oak moss absolute has been restricted by the International Fragrance Association to a maximum level of 100 ppm each.

Objectives

To compare the elicitation capacities of a traditional (sample A) and a treated (sample B) oak moss absolute containing, in total, 27 000 and 66 ppm of atranol and chloroatranol, respectively, and to investigate reactions to components of oak moss absolute separated by thin-layer chromatography (TLC).

Methods

Fifteen oak moss-allergic subjects were patch tested with serial dilutions and TLC strips of samples A and B.

Results

Fifteen subjects reacted to sample A at concentrations ≤ 2.0%, and 2 subjects reacted to sample B at 2.0% but not to lower concentrations. Among 13 subjects reacting to the TLC strip of sample A, 11 reacted to spots with retardation factor values corresponding to those of atranol and/or chloroatranol, and 11 reacted to other areas on the TLC strip. Only one subject reacted to the TLC strip of sample B.

Conclusions

The patch test reactivity of sample B was significantly lower than that of sample A. The TLC patch tests indicate the presence of sensitizers other than atranol and chloroatranol in oak moss absolute.

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