A combined test of acetylcholine-mediated vasodilation of both the forearm resistance vessels and the radial artery
Article first published online: 28 DEC 2012
© 2012 The Authors Clinical Physiology and Functional Imaging © 2012 Scandinavian Society of Clinical Physiology and Nuclear Medicine
Clinical Physiology and Functional Imaging
Volume 33, Issue 3, pages 206–210, May 2013
How to Cite
Lind, L. (2013), A combined test of acetylcholine-mediated vasodilation of both the forearm resistance vessels and the radial artery. Clinical Physiology and Functional Imaging, 33: 206–210. doi: 10.1111/cpf.12014
- Issue published online: 21 MAR 2013
- Article first published online: 28 DEC 2012
- Manuscript Accepted: 23 NOV 2012
- Manuscript Received: 24 JUL 2012
- brachial artery;
To evaluate a new combined test of endothelium-dependent vasodilation (EDV) in the forearm resistance vessel and the radial artery.
Acetylcholine (ACh) and sodium nitroprusside (SNP) infusion in the brachial artery were used to simultaneous evaluate EDV and endothelium-independent vasodilation (EIDV) in forearm resistance vessels (plethysmography) and in the radial artery (ultrasound) in 76 healthy subjects. Brachial artery ultrasound was used to assess flow-mediated vasodilation (FMD).
Acetylcholine induced a 4·5% (median value, P = 0·0010) increase in the radial artery diameter, while SNP induced a 31·2% increase in diameter (P<0·0001). While no increase in radial artery blood flow was induced by ACh (P = 0·69), an increase by 181% in radial blood flow was induced by SNP (P<0·0001).
When relating the radial artery diameter data to FMD, the change in radial artery diameter induced by ACh was significantly correlated with FMD (r = 0·35, P = 0·013). No such relationship was seen for the diameter change induced by SNP (r = −0·03, P = 0·81).
When relating the radial artery blood flow data to EDV and EIDV, the change in radial artery blood flow induced by ACh was significantly correlated with EDV in an inverse fashion (r = −0·42, P = 0·0032). No such relationship was seen for the blood flow change induced by SNP when related to EIDV (r = −0·07, P = 0·63).
Simultaneous measurements of blood flow in forearm vessels by plethysmography and in the radial artery by ultrasound during ACh infusion is a new way to assess EDV in both resistance and conduit arteries during one investigation.