Elastic three-dimensional poly (ε-caprolactone) nanofibre scaffold enhances migration, proliferation and osteogenic differentiation of mesenchymal stem cells
Article first published online: 7 DEC 2012
© 2012 Blackwell Publishing Ltd
Volume 46, Issue 1, pages 23–37, February 2013
How to Cite
Rampichová, M., Chvojka, J., Buzgo, M., Prosecká, E., Mikeš, P., Vysloužilová, L., Tvrdík, D., Kochová, P., Gregor, T., Lukáš, D. and Amler, E. (2013), Elastic three-dimensional poly (ε-caprolactone) nanofibre scaffold enhances migration, proliferation and osteogenic differentiation of mesenchymal stem cells. Cell Proliferation, 46: 23–37. doi: 10.1111/cpr.12001
- Issue published online: 7 JAN 2013
- Article first published online: 7 DEC 2012
- Manuscript Accepted: 17 AUG 2012
- Manuscript Received: 13 JUN 2012
- Academy of Sciences of the Czech Republic. Grant Numbers: AV0Z50390703, AV0Z50390512
- Grant Agency of the Academy of Sciences. Grant Number: IAA500390702
- Grant Agency of the Charles University. Grant Numbers: 96610, 97110, 330611, 384311, 164010, 626011
- Grant Agency of the Czech Republic. Grant Number: P304/10/1307
- Internal Grant Agency of the Ministry of Health of the Czech Republic. Grant Number: NT12156
- The Ministry of Education of the Czech Republic. Grant Number: ME 10145
We prepared 3D poly (ε-caprolactone) (PCL) nanofibre scaffolds and tested their use for seeding, proliferation, differentiation and migration of mesenchymal stem cell (MSCs).
Materials and methods
3D nanofibres were prepared using a special collector for common electrospinning; simultaneously, a 2D PCL nanofibre layer was prepared using a classic plain collector. Both scaffolds were seeded with MSCs and biologically tested. MSC adhesion, migration, proliferation and osteogenic differentiation were investigated.
The 3D PCL scaffold was characterized by having better biomechanical properties, namely greater elasticity and resistance against stress and strain, thus this scaffold will be able to find broad applications in tissue engineering. Clearly, while nanofibre layers of the 2D scaffold prevented MSCs from migrating through the conformation, cells infiltrated freely through the 3D scaffold. MSC adhesion to the 3D nanofibre PCL layer was also statistically more common than to the 2D scaffold (P < 0.05), and proliferation and viability of MSCs 2 or 3 weeks post-seeding, were also greater on the 3D scaffold. In addition, the 3D PCL scaffold was also characterized by displaying enhanced MSC osteogenic differentiation.
We draw the conclusion that all positive effects observed using the 3D PCL nanofibre scaffold are related to the larger fibre surface area available to the cells. Thus, the proposed 3D structure of the nanofibre layer will find a wide array of applications in tissue engineering and regenerative medicine.