Elastic three-dimensional poly (ε-caprolactone) nanofibre scaffold enhances migration, proliferation and osteogenic differentiation of mesenchymal stem cells

Authors

  • M. Rampichová,

    Corresponding author
    1. Institute of Biophysics, 2nd Faculty of Medicine, Charles University in Prague, Prague 5-Motol, Czech Republic
    • Laboratory of Tissue Engineering, Institute of Experimental Medicine, Academy of Science of the Czech Republic, Prague, Czech Republic
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  • J. Chvojka,

    1. Technical University of Liberec, Faculty of Textile Engineering, Department of Nonwoven Textiles, Czech Republic
    2. Technical University of Liberec, Institute for Nanomaterials, Advanced Technologies and Innovation, Czech Republic
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  • M. Buzgo,

    1. Laboratory of Tissue Engineering, Institute of Experimental Medicine, Academy of Science of the Czech Republic, Prague, Czech Republic
    2. Institute of Biophysics, 2nd Faculty of Medicine, Charles University in Prague, Prague 5-Motol, Czech Republic
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  • E. Prosecká,

    1. Laboratory of Tissue Engineering, Institute of Experimental Medicine, Academy of Science of the Czech Republic, Prague, Czech Republic
    2. Institute of Biophysics, 2nd Faculty of Medicine, Charles University in Prague, Prague 5-Motol, Czech Republic
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  • P. Mikeš,

    1. Technical University of Liberec, Faculty of Textile Engineering, Department of Nonwoven Textiles, Czech Republic
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  • L. Vysloužilová,

    1. Technical University of Liberec, Faculty of Textile Engineering, Department of Nonwoven Textiles, Czech Republic
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  • D. Tvrdík,

    1. Institute of Pathology, First Faculty of Medicine and General Teaching Hospital, Charles University, Prague, Czech Republic
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  • P. Kochová,

    1. European Centre of Excellence NTIS – New Technologies for Information Society, Faculty of Applied Sciences, University of West Bohemia, Pilsen, Czech Republic
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  • T. Gregor,

    1. New Technologies – Research centre, University of West Bohemia, Pilsen, Czech Republic
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  • D. Lukáš,

    1. Technical University of Liberec, Faculty of Textile Engineering, Department of Nonwoven Textiles, Czech Republic
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  • E. Amler

    1. Laboratory of Tissue Engineering, Institute of Experimental Medicine, Academy of Science of the Czech Republic, Prague, Czech Republic
    2. Institute of Biophysics, 2nd Faculty of Medicine, Charles University in Prague, Prague 5-Motol, Czech Republic
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Correspondence: M. Rampichová, Laboratory of Tissue Engineering, Institute of Experimental Medicine, Academy of Science of the Czech Republic, Videnska 1083, 142 40, Prague, Czech Republic. Tel/Fax: +420 296 442 387; E-mail: m.rampichova@biomed.cas.cz

Abstract

Objectives

We prepared 3D poly (ε-caprolactone) (PCL) nanofibre scaffolds and tested their use for seeding, proliferation, differentiation and migration of mesenchymal stem cell (MSCs).

Materials and methods

3D nanofibres were prepared using a special collector for common electrospinning; simultaneously, a 2D PCL nanofibre layer was prepared using a classic plain collector. Both scaffolds were seeded with MSCs and biologically tested. MSC adhesion, migration, proliferation and osteogenic differentiation were investigated.

Results

The 3D PCL scaffold was characterized by having better biomechanical properties, namely greater elasticity and resistance against stress and strain, thus this scaffold will be able to find broad applications in tissue engineering. Clearly, while nanofibre layers of the 2D scaffold prevented MSCs from migrating through the conformation, cells infiltrated freely through the 3D scaffold. MSC adhesion to the 3D nanofibre PCL layer was also statistically more common than to the 2D scaffold (< 0.05), and proliferation and viability of MSCs 2 or 3 weeks post-seeding, were also greater on the 3D scaffold. In addition, the 3D PCL scaffold was also characterized by displaying enhanced MSC osteogenic differentiation.

Conclusions

We draw the conclusion that all positive effects observed using the 3D PCL nanofibre scaffold are related to the larger fibre surface area available to the cells. Thus, the proposed 3D structure of the nanofibre layer will find a wide array of applications in tissue engineering and regenerative medicine.

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