Activation of proliferation and differentiation of dental follicle stem cells (DFSCs) by heat stress
Article first published online: 21 DEC 2012
© 2013 Blackwell Publishing Ltd
Volume 46, Issue 1, pages 58–66, February 2013
How to Cite
Rezai Rad, M., Wise, G.E., Brooks, H., Flanagan, M.B. and Yao, S. (2013), Activation of proliferation and differentiation of dental follicle stem cells (DFSCs) by heat stress. Cell Proliferation, 46: 58–66. doi: 10.1111/cpr.12004
- Issue published online: 7 JAN 2013
- Article first published online: 21 DEC 2012
- Manuscript Accepted: 23 AUG 2012
- Manuscript Revised: 17 AUG 2012
- Manuscript Received: 30 JUL 2012
- National Institute of Dental and Craniofacial Research. Grant Numbers: 5R03DE018998-02, 5R01DE008911-21
Adult stem cells (ASCs) remain in a slowly cycling/quiescent state under normal physiological conditions, but they can be awakened from this by certain factors, such as injury signals. Previously, our group has shown that dental follicle stem cells (DFSCs) appear to proliferate more rapidly than their non-stem cell counterparts at elevated temperatures. The study described here has aimed to (i) elucidate optimal temperature in which to culture DFSCs, (ii) determine whether elevated temperatures could enhance differentiation capability of DFSCs and (iii) characterize stem cell and osteogenic marker expression of DFSCs at elevated temperatures.
Materials and methods
DFSCs obtained from rat first molars were cultured at 37 (control), 38, 39, 40 and 41 ºC. Cell proliferation was evaluated by Alamar blue reduction assay and mean numbers of viable dissociated cells. Osteogenic differentiation was evaluated after 7 or 14 days osteogenic induction. Expression of selected marker genes was also assessed during proliferation and differentiation of the cells.
Increased cell proliferation was seen at heat-stress temperatures of 38º, 39º and 40 ºC. DFSCs revealed maximal osteogenesis when cultured at 39 and 40 ºC. Moreover, some stem cell and osteogensis-associated markers had elevated expression in heat-stress conditions.
Under determined heat-stress conditions, DFSCs increased their proliferation, osteogenic differentiation and expression of some marker genes. Thus, it is likely that elevated temperature could serve as a factor to activate adult stem cells.