In vitro cytotoxicity of Gymnema montanum in human leukaemia HL-60 cells; induction of apoptosis by mitochondrial membrane potential collapse
Version of Record online: 21 MAY 2013
© 2013 Blackwell Publishing Ltd
Volume 46, Issue 3, pages 263–271, June 2013
How to Cite
Ramkumar, K. M., Manjula, C., Elango, B., Krishnamurthi, K., Saravana Devi, S. and Rajaguru, P. (2013), In vitro cytotoxicity of Gymnema montanum in human leukaemia HL-60 cells; induction of apoptosis by mitochondrial membrane potential collapse. Cell Proliferation, 46: 263–271. doi: 10.1111/cpr.12033
- Issue online: 21 MAY 2013
- Version of Record online: 21 MAY 2013
- Manuscript Accepted: 19 JAN 2013
- Manuscript Received: 5 NOV 2012
Gymnema montanum Hook, an Indian Ayurvedic medicinal plant, is used traditionally to treat a variety of ailments. Here, we report anti-cancer effects and molecular mechanisms of ethanolic extract of G. montanum (GLEt) on human leukaemia HL-60 cells, compared to peripheral blood mononuclear cells.
Materials and methods
HL-60 cells were treated with different concentrations of GLEt (10–50 μg/ml) and cytotoxicity was assessed by MTT assay. Levels of lipid peroxidation, antioxidants, mitochondrial membrane potential and caspase-3 were measured. Further, apoptosis was studied using annexin-V staining and the cell cycle was analyzed by flow cytometry.
GLEt had a potent cytotoxic effect on HL-60 cells (IC50-20 μg/ml), yet was not toxic to normal peripheral blood mononuclear cells. Exposure of HL-60 cells to GLEt led to elevated levels of malonaldehyde formation, but to reduced glutathione, superoxide dismutase, catalase and glutathione peroxidase activities (P < 0.05). Induction of apoptosis was confirmed by observing annexin-V positive cells, associated with loss of mitochondrial membrane potential. Cell cycle arrest at G0/G1 was observed in GLEt-treated HL-60 cells, indicating its potential at inducing their apoptosis.
Findings of the present study suggest that G. montanum induced apoptosis in the human leukaemic cancer cells, mediated by collapse of mitochondrial membrane potential, generation of reactive oxygen species and depletion of intracellular antioxidant potential.